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Status
Public on Jan 22, 2017
Title
Aire KO DMSO 3
Sample type
RNA
Source name
Thymus
Organism
Mus musculus
Characteristics
age: 6 Weeks Old Sex: Female strain: C57Bl/6 genotype: Aire-/- cells: MECs treatment: DMSO amplification technique: Ambion WT Expression Kit separation method: Dissection / Trimming
Extracted molecule
total RNA
Extraction protocol
TriZol
Label
Biotin
Label protocol
Affymetrix GeneChip WT Terminal Labeling and Controls Kit
Hybridization protocol
Ambion WT Expression Kit
Scan protocol
Affymetrix GeneChip Scanner
Data processing
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Dec 16, 2016
Last update date
Jan 22, 2017
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu
Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL6246
Series (2)
GSE92509
Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654
Aire, guardian of immunological tolerance, binds to and activates super-enhancers
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
29.2141
10344622
376.0732
10344624
1551.0644
10344633
1580.9212
10344637
726.8718
10344653
47.6679
10344658
404.9439
10344674
26.7429
10344679
170.7699
10344707
785.601
10344713
681.9255
10344723
159.3253
10344725
185.3951
10344741
1552.5806
10344743
70.0467
10344750
1684.7747
10344772
83.0395
10344789
421.9584
10344797
619.4529
10344799
549.9658
Total number of rows: 24922 Table truncated, full table size 433 Kbytes .Supplementary file
Size
Download
File type/resource
GSM2430934_EA07068_371488_MOGENE-1_0-ST-V1_MECHI.AIRE.DMSO_4.CEL.gz
4.4 Mb
(ftp) (http)
CEL
Processed data included within Sample table