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Sample GSM2425632 Query DataSets for GSM2425632
Status Public on Oct 02, 2017
Title C3640
Sample type SRA
Source name Canine fetal gonads, day 39
Organism Canis lupus familiaris
Characteristics developmental stage: day 39 embryo
tissue: gonads
Sex: male
genotype: (G+/-)
Extracted molecule total RNA
Extraction protocol Gonads were isolated by dissection of fetal tissues.
We prepared PRO-seq libraries as described in Kwak et. al. (2013) Science.
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina NextSeq 500
Description nascent RNA
Data processing Library strategy: PRO-Seq
We removed exact duplicate reads and trimmed a 6bp random hexamer at the 5' end of each read.
We trimmed adapter sequences and mapped PRO-seq data using BWA.
Data was converted into bigWig format.
Genome_build: canFam3
Supplementary_files_format_and_content: PRO-seq data are provided in standard bigWig and raw fastq formats.
Submission date Dec 13, 2016
Last update date May 15, 2019
Contact name Charles G Danko
Organization name Cornell University
Department Baker Institute
Lab Danko Lab
Street address Hungerford Hill Rd
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
Platform ID GPL21400
Series (1)
GSE92318 RSPO1 downregulation and a CFA9 Insertion are Associated with Inherited XX DSD in a Canine Model
BioSample SAMN06134806
SRA SRX2417269

Supplementary file Size Download File type/resource 26.1 Mb (ftp)(http) BW 26.4 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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