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Sample GSM2424345 Query DataSets for GSM2424345
Status Public on Feb 06, 2017
Title Trsp-Ins1KO hypothlamus 2
Sample type RNA
Source name Hypothlamus_Trsp-Ins1KO
Organism Mus musculus
Characteristics strain background: C57BL/6J
gender: Male
age: 16 weeks
genotype/variation: TrspFlox/Flox::Ins1-Cre (Trsp-Ins1KO)
tissue: Hypothlamus
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Sepazol-RNA I Super G RNA solution (Nakalai Tesque).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using theLow Input Quick Amp Labeling Kits (Agilent) according to the manufacturer's protocol.
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Data processing The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
Submission date Dec 11, 2016
Last update date Feb 06, 2017
Contact name Akira Uruno
Phone 81-22-717-8085
Organization name Tohoku University Graduate School of Medicine
Street address 2-1 Seiryo-cho, Aoba-ku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
Platform ID GPL11202
Series (2)
GSE92235 Hypothalamic oxidative stress provokes leptin and insulin resistance [hypothlamus]
GSE92236 Hypothalamic oxidative stress provokes leptin and insulin resistance

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner -0.32622194
DarkCorner -0.22034073
A_55_P1989846 0.217525
A_55_P1991598 0.003925324
A_55_P2022211 -0.23320293
A_55_P1980764 -0.025384426
A_55_P1964375 -0.009503365
A_51_P128876 0.29054546
A_55_P2121042 0.00256443
A_52_P219230 0.6442528
A_51_P207591 1.674159
A_55_P2131920 -0.1186471
A_55_P2404223 0.28402615
A_55_P2101944 -0.29354143
A_52_P358860 -0.041137695
A_51_P119031 -0.22876024
A_51_P309854 -0.1147933
A_51_P343900 0.14251757
A_51_P234359 -0.18025923
A_51_P487813 -0.1257968

Total number of rows: 39485

Table truncated, full table size 972 Kbytes.

Supplementary file Size Download File type/resource
GSM2424345_6_Trsp-Ins1KO_hypothlamus_2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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