GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM2424344 Query DataSets for GSM2424344
Status Public on Feb 06, 2017
Title Trsp-Ins1KO hypothlamus 1
Sample type RNA
Source name Hypothlamus_Trsp-Ins1KO
Organism Mus musculus
Characteristics strain background: C57BL/6J
gender: Male
age: 16 weeks
genotype/variation: TrspFlox/Flox::Ins1-Cre (Trsp-Ins1KO)
tissue: Hypothlamus
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Sepazol-RNA I Super G RNA solution (Nakalai Tesque).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using theLow Input Quick Amp Labeling Kits (Agilent) according to the manufacturer's protocol.
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Data processing The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
Submission date Dec 11, 2016
Last update date Feb 06, 2017
Contact name Akira Uruno
Phone 81-22-717-8085
Organization name Tohoku University Graduate School of Medicine
Street address 2-1 Seiryo-cho, Aoba-ku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
Platform ID GPL11202
Series (2)
GSE92235 Hypothalamic oxidative stress provokes leptin and insulin resistance [hypothlamus]
GSE92236 Hypothalamic oxidative stress provokes leptin and insulin resistance

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.079435825
DarkCorner 0.07944107
A_55_P1989846 -0.005423069
A_55_P1991598 0.26443243
A_55_P2022211 0.009015083
A_55_P1980764 -0.020638943
A_55_P1964375 -0.009081841
A_51_P128876 -0.21253967
A_55_P2121042 0.25574255
A_52_P219230 -0.17488575
A_51_P207591 0.30987692
A_55_P2131920 -0.16719866
A_55_P2404223 0.21845245
A_55_P2101944 -0.019164562
A_52_P358860 -0.0362854
A_51_P119031 -0.21591616
A_51_P309854 -0.24410772
A_51_P343900 0.13553667
A_51_P234359 -0.46993113
A_51_P487813 -0.16734076

Total number of rows: 39485

Table truncated, full table size 976 Kbytes.

Supplementary file Size Download File type/resource
GSM2424344_5_Trsp-Ins1KO_hypothlamus_1.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap