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Sample GSM2424343 Query DataSets for GSM2424343
Status Public on Feb 06, 2017
Title Trsp-RIPKO hypothlamus 2
Sample type RNA
 
Source name Hypothlamus_Trsp-RIPKO
Organism Mus musculus
Characteristics strain background: C57BL/6J
gender: Male
age: 16 weeks
genotype/variation: TrspFlox/Flox::RIP-Cre (Trsp-RIPKO)
tissue: Hypothlamus
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Sepazol-RNA I Super G RNA solution (Nakalai Tesque).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using theLow Input Quick Amp Labeling Kits (Agilent) according to the manufacturer's protocol.
 
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Data processing The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
 
Submission date Dec 11, 2016
Last update date Feb 06, 2017
Contact name Akira Uruno
E-mail(s) aurno02821@gmail.com
Phone 81-22-717-8085
Organization name Tohoku University Graduate School of Medicine
Street address 2-1 Seiryo-cho, Aoba-ku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
 
Platform ID GPL11202
Series (2)
GSE92235 Hypothalamic oxidative stress provokes leptin and insulin resistance [hypothlamus]
GSE92236 Hypothalamic oxidative stress provokes leptin and insulin resistance

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner -0.079435825
DarkCorner -0.20768356
A_55_P1989846 0.1828847
A_55_P1991598 -0.00392437
A_55_P2022211 -0.009015083
A_55_P1980764 -0.1328783
A_55_P1964375 0.016073227
A_51_P128876 -0.58098507
A_55_P2121042 -0.00256443
A_52_P219230 -0.43136597
A_51_P207591 -1.6615934
A_55_P2131920 -0.028371334
A_55_P2404223 0.013713837
A_55_P2101944 -0.303267
A_52_P358860 0.03376293
A_51_P119031 0.26079988
A_51_P309854 0.1147933
A_51_P343900 0.059258938
A_51_P234359 0.5617051
A_51_P487813 0.42784643

Total number of rows: 39485

Table truncated, full table size 969 Kbytes.




Supplementary file Size Download File type/resource
GSM2424343_4_Trsp-RIPKO_hypothlamus_2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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