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Sample GSM2424340 Query DataSets for GSM2424340
Status Public on Feb 06, 2017
Title TrspFF hypothlamus 1
Sample type RNA
 
Source name Hypothlamus_TrspFF
Organism Mus musculus
Characteristics strain background: C57BL/6J
gender: Male
age: 16 weeks
genotype/variation: TrspFlox/Flox (TrspFF)
tissue: Hypothlamus
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Sepazol-RNA I Super G RNA solution (Nakalai Tesque).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using theLow Input Quick Amp Labeling Kits (Agilent) according to the manufacturer's protocol.
 
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Data processing The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
 
Submission date Dec 11, 2016
Last update date Feb 06, 2017
Contact name Akira Uruno
E-mail(s) aurno02821@gmail.com
Phone 81-22-717-8085
Organization name Tohoku University Graduate School of Medicine
Street address 2-1 Seiryo-cho, Aoba-ku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
 
Platform ID GPL11202
Series (2)
GSE92235 Hypothalamic oxidative stress provokes leptin and insulin resistance [hypothlamus]
GSE92236 Hypothalamic oxidative stress provokes leptin and insulin resistance

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 0.4105525
DarkCorner 0.011797905
A_55_P1989846 -0.23594618
A_55_P1991598 -0.061217308
A_55_P2022211 0.32672596
A_55_P1980764 0.29799032
A_55_P1964375 -0.006546021
A_51_P128876 0.7101517
A_55_P2121042 -0.032569885
A_52_P219230 -0.45679283
A_51_P207591 -0.30987692
A_55_P2131920 0.030268192
A_55_P2404223 -0.016457558
A_55_P2101944 0.15369654
A_52_P358860 0.034085274
A_51_P119031 0.121008396
A_51_P309854 0.11814356
A_51_P343900 -0.22053957
A_51_P234359 0.18025923
A_51_P487813 0.1257968

Total number of rows: 39485

Table truncated, full table size 965 Kbytes.




Supplementary file Size Download File type/resource
GSM2424340_1_TrspFF_hypothlamus_1.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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