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Sample GSM2424337 Query DataSets for GSM2424337
Status Public on Feb 06, 2017
Title Trsp-RIPKO islets 2
Sample type RNA
 
Source name Islets, Trsp-RIPKO
Organism Mus musculus
Characteristics strain background: ICR
gender: Male
age: 16 weeks
genotype/variation: TrspFlox/Flox::RIP-Cre (Trsp-RIPKO)
tissue: pancreatic islets
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Sepazol-RNA I Super G RNA solution (Nakalai Tesque).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using theLow Input Quick Amp Labeling Kits (Agilent) according to the manufacturer's protocol.
 
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Description Gene expression in the islets of Trsp-RIPKO mouse
Data processing The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
 
Submission date Dec 11, 2016
Last update date Feb 06, 2017
Contact name Akira Uruno
E-mail(s) aurno02821@gmail.com
Phone 81-22-717-8085
Organization name Tohoku University Graduate School of Medicine
Street address 2-1 Seiryo-cho, Aoba-ku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
 
Platform ID GPL11202
Series (2)
GSE92234 Hypothalamic oxidative stress provokes leptin and insulin resistance [Islets]
GSE92236 Hypothalamic oxidative stress provokes leptin and insulin resistance

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner -0.22289515
DarkCorner -0.31488943
A_55_P1989846 0.81005764
A_55_P1991598 -0.42917252
A_55_P2022211 0.5347872
A_55_P1980764 -0.41259384
A_55_P1964375 -0.13687849
A_51_P128876 -0.040656805
A_55_P2121042 -0.38933945
A_52_P219230 -0.38184834
A_51_P207591 -0.32806873
A_55_P2131920 1.1182215
A_55_P2404223 -0.0889802
A_55_P2101944 0.67117596
A_52_P358860 -0.680681
A_51_P119031 0.034336567
A_51_P309854 0.374398
A_51_P343900 -0.18410206
A_51_P234359 0.002130508
A_51_P487813 0.41001868

Total number of rows: 39485

Table truncated, full table size 968 Kbytes.




Supplementary file Size Download File type/resource
GSM2424337_4_Trsp-RIPKO_islets_2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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