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Sample GSM2424336 Query DataSets for GSM2424336
Status Public on Feb 06, 2017
Title Trsp-RIPKO islets 1
Sample type RNA
 
Source name Islets, Trsp-RIPKO
Organism Mus musculus
Characteristics strain background: ICR
gender: Male
age: 16 weeks
genotype/variation: TrspFlox/Flox::RIP-Cre (Trsp-RIPKO)
tissue: pancreatic islets
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Sepazol-RNA I Super G RNA solution (Nakalai Tesque).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using theLow Input Quick Amp Labeling Kits (Agilent) according to the manufacturer's protocol.
 
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Description Gene expression in the islets of Trsp-RIPKO mouse
Data processing The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
 
Submission date Dec 11, 2016
Last update date Feb 06, 2017
Contact name Akira Uruno
E-mail(s) aurno02821@gmail.com
Phone 81-22-717-8085
Organization name Tohoku University Graduate School of Medicine
Street address 2-1 Seiryo-cho, Aoba-ku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
 
Platform ID GPL11202
Series (2)
GSE92234 Hypothalamic oxidative stress provokes leptin and insulin resistance [Islets]
GSE92236 Hypothalamic oxidative stress provokes leptin and insulin resistance

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 0.050639153
DarkCorner 0.058665752
A_55_P1989846 -0.18066025
A_55_P1991598 -0.08362532
A_55_P2022211 0.40397406
A_55_P1980764 -0.0725956
A_55_P1964375 -0.00459671
A_51_P128876 0.040656805
A_55_P2121042 -0.05443096
A_52_P219230 -0.04774475
A_51_P207591 -0.064186096
A_55_P2131920 1.0863061
A_55_P2404223 0.11361027
A_55_P2101944 0.89021015
A_52_P358860 -0.2160089
A_51_P119031 -0.08539772
A_51_P309854 0.4613788
A_51_P343900 -0.16420603
A_51_P234359 -0.002130508
A_51_P487813 0.115676165

Total number of rows: 39485

Table truncated, full table size 969 Kbytes.




Supplementary file Size Download File type/resource
GSM2424336_3_Trsp-RIPKO_islets_1.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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