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Sample GSM2424335 Query DataSets for GSM2424335
Status Public on Feb 06, 2017
Title TrspFF islets 2
Sample type RNA
Source name Islets, TrspFF
Organism Mus musculus
Characteristics strain background: ICR
gender: Male
age: 16 weeks
genotype/variation: TrspFlox/Flox (TrspFF)
tissue: pancreatic islets
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Sepazol-RNA I Super G RNA solution (Nakalai Tesque).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using theLow Input Quick Amp Labeling Kits (Agilent) according to the manufacturer's protocol.
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Description Gene expression in the islets of TrspFF mouse
Data processing The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
Submission date Dec 11, 2016
Last update date Feb 06, 2017
Contact name Akira Uruno
Phone 81-22-717-8085
Organization name Tohoku University Graduate School of Medicine
Street address 2-1 Seiryo-cho, Aoba-ku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
Platform ID GPL11202
Series (2)
GSE92234 Hypothalamic oxidative stress provokes leptin and insulin resistance [Islets]
GSE92236 Hypothalamic oxidative stress provokes leptin and insulin resistance

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.08568859
DarkCorner 0.7782035
A_55_P1989846 0.10008049
A_55_P1991598 0.88358736
A_55_P2022211 0.009934902
A_55_P1980764 0.8790746
A_55_P1964375 0.00459671
A_51_P128876 -0.46965194
A_55_P2121042 0.8734298
A_52_P219230 0.87145185
A_51_P207591 0.56136894
A_55_P2131920 -0.30632877
A_55_P2404223 -0.21228147
A_55_P2101944 0.20497131
A_52_P358860 -0.02833438
A_51_P119031 -0.1295805
A_51_P309854 -0.030764818
A_51_P343900 0.1810584
A_51_P234359 -1.382515
A_51_P487813 -0.039207697

Total number of rows: 39485

Table truncated, full table size 953 Kbytes.

Supplementary file Size Download File type/resource
GSM2424335_2_TrspFF_islets_2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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