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Sample GSM2424334 Query DataSets for GSM2424334
Status Public on Feb 06, 2017
Title TrspFF islets 1
Sample type RNA
Source name Islets, TrspFF
Organism Mus musculus
Characteristics strain background: ICR
gender: Male
age: 16 weeks
genotype/variation: TrspFlox/Flox (TrspFF)
tissue: pancreatic islets
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Sepazol-RNA I Super G RNA solution (Nakalai Tesque).
Label Cy3
Label protocol RNA samples were labeled with Cy3 using theLow Input Quick Amp Labeling Kits (Agilent) according to the manufacturer's protocol.
Hybridization protocol Cy3-labeled cRNA were mixed and fragmented using the Agilent In Situ Hybridization Kit protocol. Hybridizations were performed for 16 hours according to the Agilent 60-mer oligo microarray processing protocol prior to washing.
Scan protocol Slides were scanned with an Agilent DNA Microarray Scanner.
Description Gene expression in the islets of TrspFF mouse
Data processing The data were normalized by percentile shift and filtered by flag, using GeneSpring GX software (Silicon Genetics).
Submission date Dec 11, 2016
Last update date Feb 06, 2017
Contact name Akira Uruno
Phone 81-22-717-8085
Organization name Tohoku University Graduate School of Medicine
Street address 2-1 Seiryo-cho, Aoba-ku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
Platform ID GPL11202
Series (2)
GSE92234 Hypothalamic oxidative stress provokes leptin and insulin resistance [Islets]
GSE92236 Hypothalamic oxidative stress provokes leptin and insulin resistance

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 0.12300682
DarkCorner 0.59747934
A_55_P1989846 -0.10008049
A_55_P1991598 0.6814127
A_55_P2022211 -0.13390684
A_55_P1980764 0.6749387
A_55_P1964375 -0.13088036
A_51_P128876 -0.24408221
A_55_P2121042 0.6668825
A_52_P219230 0.6649742
A_51_P207591 0.3878398
A_55_P2131920 -0.5126662
A_55_P2404223 -0.074807644
A_55_P2101944 -0.25171947
A_52_P358860 0.49977946
A_51_P119031 -0.034336567
A_51_P309854 -0.24531579
A_51_P343900 0.18556738
A_51_P234359 -0.8073275
A_51_P487813 0.039207697

Total number of rows: 39485

Table truncated, full table size 957 Kbytes.

Supplementary file Size Download File type/resource
GSM2424334_1_TrspFF_islets_1.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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