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Sample GSM2420634 Query DataSets for GSM2420634
Status Public on Dec 31, 2016
Title HEK293T_clone1_SC3
Sample type SRA
Source name HEK293T single cell
Organism Homo sapiens
Characteristics cell line: HEK293T
Growth protocol HEK293T cells were grown in DMEM/High Glucose medium (ThermoFisher Scientific) with 10% FBS (Life Technologies) and 1% Penicillin-Streptomycin (Life Technologies). Cells were passed every 2-3 days.
Extracted molecule total RNA
Extraction protocol cDNA libraries were prepared for sequencing using standard Illumina Truseq protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Data processing The raw sequencing data was trimmed using skewer package.
The reads were mapped to the transcriptome using TOPHAT
A Python program using HT-seq package was written to analyze the barcode information
Genome_build: Hg19
Supplementary_files_format_and_content: txt files include the read count and barcode count for exon-based and intron-based analyses
Submission date Dec 09, 2016
Last update date May 15, 2019
Contact name Kuanwei Sheng
Organization name Baylor College of Medicine
Department Molecular and Human Genetics
Lab Zong Lab
Street address ONE BAYLOR PALAZA
State/province TX
ZIP/Postal code 77030
Country USA
Platform ID GPL18573
Series (1)
GSE78968 Effective Detection of Variation in Single Cell Transcriptome using MATQ-seq
BioSample SAMN06127906
SRA SRX2408876

Supplementary file Size Download File type/resource
GSM2420634_HEK293T_clone1_SC3.stats.txt.gz 339.5 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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