 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 18, 2017 |
| Title |
819b_A2 |
| Sample type |
SRA |
| |
|
| Source name |
Mouse back skin
|
| Organism |
Mus musculus |
| Characteristics |
Sex: Mixed
age: P21
strain: Lgr5-eGFPiresCreER;R26-tdTomato
sorting markers: GFP, tdTomato, integrin α6, integrin b1, CD34, Pcadherin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single bulge hair follicle stem cells, hair germ cells and basal hair bulb cells at indicated age were FACS-purified, collected into 96 well plates containing lysis buffer and stored in -80oC
Single cells were lyzed by incubating at 72oC for 3 min. Revers transcritpion reaction and whole transcriptome amplification were perforemd according to Smart-seq2 protocol. The amplified cDNA libraries were purified by Ampure XP beads (Beckman Coulter), and tagmentation reaction was perforemd by Illumina Nextera XT DNA Library Prep Kit. The libraries were pooled and sequecned on Illumina Nextseq 500 with mid-output 75cycles paired-end mode.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Tel_Ana1_Ana2_bulge_HG_basal_HB_TPM.txt
mm10
tab-delimited text
|
| Data processing |
Alignment: Sequence reads were obtained and mapped to the mouse genome (mm10) using the Bowtie2 program (version 2.2.9).
The expression values of each gene were quantified as transcript per million (TPM) using RSEM (v1.2.30)
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include TPM values for each Sample
|
| |
|
| Submission date |
Dec 04, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Hanseul Yang |
| E-mail(s) |
hyang01@rockefeller.edu
|
| Organization name |
Rockefeller University
|
| Lab |
Fuchs lab
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE90848 |
Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices (SC RNA-Seq) |
| GSE90850 |
Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices |
|
| Relations |
| BioSample |
SAMN06108229 |
| SRA |
SRX2393726 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
