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Sample GSM2415054 Query DataSets for GSM2415054
Status Public on Apr 18, 2017
Title Ana-VI_HFSC_RNAseq_rep3
Sample type SRA
 
Source name Mouse back skin
Organism Mus musculus
Characteristics Sex: Mixed
age: P30
strain: Lhx2-GFP (CD1)
sorting markers: GFP, integrin α6, integrin b1, CD34, Sca-1, Pcadherin
Extracted molecule total RNA
Extraction protocol Mouse hair bulb cells were FACS-purified from P30 K14-H2BGFP mice.
FACS-isolated cells were directly lysed into TRIzol LS (Invitrogen). Total RNA was purified using the Direct-zol RNA MiniPrep kit (Zymo Research) per manufacturer’s instructions. Quality of the RNA for sequencing was determined using an Agilent 2100 Bioanalyzer; all samples used had RNA integrity numbers >9. Library preparation using the Illumina TrueSeq mRNA sample preparation kit was performed at the Weill Cornell Medical College Genomic Core facility (New York), and RNAs were paired-end sequenced on Illumina HiSeq 2000 machines.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Alignment: Sequence reads were obtained and mapped to the mouse genome (mm10) using the Bowtie2 program (version 2.2.9).
The expression values of each gene were quantified as transcript per million (TPM) using RSEM (v1.2.30)
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include TPM values for each Sample
 
Submission date Dec 04, 2016
Last update date May 15, 2019
Contact name Hanseul Yang
E-mail(s) hyang01@rockefeller.edu
Organization name Rockefeller University
Lab Fuchs lab
Street address 1230 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL13112
Series (2)
GSE90847 Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices (bulk RNA-Seq)
GSE90850 Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices
Relations
BioSample SAMN06107608
SRA SRX2393716

Supplementary file Size Download File type/resource
GSM2415054_Bulge_2324.genes.results.txt.gz 1.5 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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