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Status |
Public on Apr 18, 2017 |
Title |
Ana-VI_HFSC_RNAseq_rep2 |
Sample type |
SRA |
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Source name |
Mouse back skin
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Organism |
Mus musculus |
Characteristics |
Sex: Mixed age: P30 strain: Lhx2-GFP (CD1) sorting markers: GFP, integrin α6, integrin b1, CD34, Sca-1, Pcadherin
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Extracted molecule |
total RNA |
Extraction protocol |
Mouse hair bulb cells were FACS-purified from P30 K14-H2BGFP mice. FACS-isolated cells were directly lysed into TRIzol LS (Invitrogen). Total RNA was purified using the Direct-zol RNA MiniPrep kit (Zymo Research) per manufacturer’s instructions. Quality of the RNA for sequencing was determined using an Agilent 2100 Bioanalyzer; all samples used had RNA integrity numbers >9. Library preparation using the Illumina TrueSeq mRNA sample preparation kit was performed at the Weill Cornell Medical College Genomic Core facility (New York), and RNAs were paired-end sequenced on Illumina HiSeq 2000 machines.
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
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Data processing |
Alignment: Sequence reads were obtained and mapped to the mouse genome (mm10) using the Bowtie2 program (version 2.2.9). The expression values of each gene were quantified as transcript per million (TPM) using RSEM (v1.2.30) Genome_build: mm10 Supplementary_files_format_and_content: tab-delimited text files include TPM values for each Sample
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Submission date |
Dec 04, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Hanseul Yang |
E-mail(s) |
hyang01@rockefeller.edu
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Organization name |
Rockefeller University
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Lab |
Fuchs lab
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Street address |
1230 York Avenue
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City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
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Platform ID |
GPL13112 |
Series (2) |
GSE90847 |
Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices (bulk RNA-Seq) |
GSE90850 |
Epithelial-Mesenchymal Micro-Niches Govern Stem Cell Lineage Choices |
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Relations |
BioSample |
SAMN06107609 |
SRA |
SRX2393715 |
Supplementary file |
Size |
Download |
File type/resource |
GSM2415053_Bulge_2122.genes.results.txt.gz |
1.5 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
Processed data are available on Series record |
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