|
| Status |
Public on Sep 30, 2017 |
| Title |
Thy1 RGCs, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
retinal cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
developmental age: postnatal day 6
tissue: retina
cell type: Thy1+ retinal cells
neuron subtype: Retinal ganglion cells (RGCs)
|
| Treatment protocol |
Papain-dissociated retinal cell suspension.
|
| Growth protocol |
Postnatal day 6 mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PicoPure RNA Isolation Kit (MDS).
|
| Label |
biotin
|
| Label protocol |
MessageAmp II-biotin system (Ambion). Two rounds of amplification were used.
|
| |
|
| Hybridization protocol |
20ug of aRNA were fragmented and hybridized according to standard Affymetrix protocols for 16 h at 45 degrees C. Arrays were washed and stained in Affymetrix Fluidics Station 450.
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Thy1-a
|
| Data processing |
Data were analyzed in dChip
|
| |
|
| Submission date |
Nov 29, 2016 |
| Last update date |
Sep 30, 2017 |
| Contact name |
Yi-Rong Peng |
| E-mail(s) |
yirongpeng@gmail.com
|
| Organization name |
University of California, Los Angeles
|
| Department |
Department of Ophthalmology
|
| Lab |
Yi-Rong Peng
|
| Street address |
100 Stein Plaza Driveway, B-133
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE90648 |
A gene expression database for retinal neuron subtypes |
| GSE90673 |
Expression profiles of retinal neuronal cells |
|
