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Sample GSM2387439 Query DataSets for GSM2387439
Status Public on Dec 31, 2018
Title VR15 ESC_rep2
Sample type RNA
 
Source name embryonic stem cell
Organism Mus musculus
Characteristics strain/background: Embryonic stem cell (Vasa::RFP Tg)
cell line: VR15
gender: male
cell type: embryonic stem cell
Extracted molecule total RNA
Extraction protocol Total RNA was purified using the RNeasy micro kit according to the manufacturer's instructions.
Label Cy3
Label protocol Cy3-labeled cRNA samples were prepared using the Agilent Low Input Quick Amp Labeling Kit (one-color) (5190-2305) and RNA Spike-In Kit (one-color) (5188-5282) according to the manufacturer's instructions. Input was 100 ng of total RNA from each sample.
 
Hybridization protocol 1.65ug of Cy3-labelled cRNAs from each sample were fragmented and hybridized to microarrays according to the manufacturer's instructions using the Gene Expression Hybridization kit (Agilent 5188-5242) and Agilent hybridization oven. After hybridization, microarrays were washed using the Gene Expression Wash Pack (Agilent 5188-5327).
Scan protocol The microarrays were scanned using the Agilent DNA microarray scanner (Agilent G2505C). The spot signals were quantified using Feature Extraction software.
Description Gene expression of mouse male ESCs (Vasa::RFP Tg).
Replicate2
Data processing Data was normalized using 75th percentile shift and analyzed by GeneSpring GX (Agilent).
 
Submission date Nov 09, 2016
Last update date Dec 31, 2018
Contact name Yohei Hayashi
E-mail(s) yohei.hayashi.e2@tohoku.ac.jp
Phone +81-22-717-8572
Organization name Tohoku University
Department Institute of Development, Aging and Cancer
Lab Cell Resource Center for Biomedical Research
Street address 4-1 Seiryo-machi, Aobaku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
 
Platform ID GPL11202
Series (1)
GSE89738 Characteristic energy metabolism in mouse primordial germ cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity (baseline of the data was median transformed)

Data table
ID_REF VALUE
GE_BrightCorner 0.004683018
DarkCorner -0.20342302
A_55_P1989846 -0.8200445
A_55_P1991598 -0.23071623
A_55_P2022211 -1.1699424
A_55_P1980764 0.44262457
A_55_P1964375 -0.052333355
A_51_P128876 1.2627048
A_55_P2121042 -0.23342752
A_52_P219230 -0.23377323
A_51_P207591 4.0554624
A_55_P2131920 -0.34295344
A_55_P2404223 0.59355426
A_55_P2101944 0.4843893
A_52_P358860 0.7029228
A_51_P119031 0.23439789
A_51_P309854 -0.25501013
A_51_P343900 0.48549128
A_51_P234359 -2.2780104
A_51_P487813 -0.6535821

Total number of rows: 39485

Table truncated, full table size 954 Kbytes.




Supplementary file Size Download File type/resource
GSM2387439_US80703200_252665518008_S02_GE1_107_Sep09_1_3_VR15b.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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