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Sample GSM2387436 Query DataSets for GSM2387436
Status Public on Dec 31, 2018
Title E13.5 Soma_rep2
Sample type RNA
 
Source name gonadal somatic cell
Organism Mus musculus
Characteristics strain/background: MCH x C57BL6/J (Oct5<delta>PE-GFP Tg)
developmental stage: E13.5
gender: male
cell type: gonadal somatic cell
Extracted molecule total RNA
Extraction protocol Total RNA was purified using the RNeasy micro kit according to the manufacturer's instructions.
Label Cy3
Label protocol Cy3-labeled cRNA samples were prepared using the Agilent Low Input Quick Amp Labeling Kit (one-color) (5190-2305) and RNA Spike-In Kit (one-color) (5188-5282) according to the manufacturer's instructions. Input was 100 ng of total RNA from each sample.
 
Hybridization protocol 1.65ug of Cy3-labelled cRNAs from each sample were fragmented and hybridized to microarrays according to the manufacturer's instructions using the Gene Expression Hybridization kit (Agilent 5188-5242) and Agilent hybridization oven. After hybridization, microarrays were washed using the Gene Expression Wash Pack (Agilent 5188-5327).
Scan protocol The microarrays were scanned using the Agilent DNA microarray scanner (Agilent G2505C). The spot signals were quantified using Feature Extraction software.
Description Gene expression of Somas from mouse E13.5 male embryo genital ridges.
Replicate2
Data processing Data was normalized using 75th percentile shift and analyzed by GeneSpring GX (Agilent).
 
Submission date Nov 09, 2016
Last update date Dec 31, 2018
Contact name Yohei Hayashi
E-mail(s) yohei.hayashi.e2@tohoku.ac.jp
Phone +81-22-717-8572
Organization name Tohoku University
Department Institute of Development, Aging and Cancer
Lab Cell Resource Center for Biomedical Research
Street address 4-1 Seiryo-machi, Aobaku
City Sendai
State/province Miyagi
ZIP/Postal code 980-8575
Country Japan
 
Platform ID GPL11202
Series (1)
GSE89738 Characteristic energy metabolism in mouse primordial germ cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity (baseline of the data was median transformed)

Data table
ID_REF VALUE
GE_BrightCorner -0.06875467
DarkCorner -0.37819147
A_55_P1989846 0.61459875
A_55_P1991598 -0.40972662
A_55_P2022211 1.1699424
A_55_P1980764 0.000408173
A_55_P1964375 -0.003705502
A_51_P128876 -1.4313831
A_55_P2121042 -0.41640902
A_52_P219230 -0.41763306
A_51_P207591 -4.6271763
A_55_P2131920 0.1950891
A_55_P2404223 0.026732683
A_55_P2101944 -0.100595474
A_52_P358860 -0.7029228
A_51_P119031 -0.23439789
A_51_P309854 0.44458246
A_51_P343900 -0.006549358
A_51_P234359 2.4442034
A_51_P487813 0.448606

Total number of rows: 39485

Table truncated, full table size 955 Kbytes.




Supplementary file Size Download File type/resource
GSM2387436_US80703200_252665518007_S02_GE1_107_Sep09_1_2_Soma2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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