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Sample GSM2386196 Query DataSets for GSM2386196
Status Public on Aug 01, 2017
Title Batf-/- Treg_1
Sample type RNA
Source name Batf-/- Treg cells
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: lymph node and spleen
cell type: hCD2(+)CD4(+) cells
sorted from: FoxP3hCD2.Batf-/- mice
Treatment protocol None
Growth protocol ex vivo isolated cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
Label biotin
Label protocol RNA was subjected to biotinylated cDNA synthesis by using GeneChip 3'IVT Express Kit.
Hybridization protocol The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description freshly sorted from FoxP3hCD2.Batf-/- mice
Data processing Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
Submission date Nov 08, 2016
Last update date Aug 01, 2017
Contact name Shohei Hori
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platform ID GPL1261
Series (2)
GSE89656 Expression data from BATF-deficient Treg cells
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
AFFX-BioB-5_at 0.028526306
AFFX-BioB-M_at 0.14321518
AFFX-BioB-3_at 0.071445465
AFFX-BioC-5_at -0.011392593
AFFX-BioC-3_at -0.001621246
AFFX-BioDn-5_at -0.024766922
AFFX-BioDn-3_at -0.044612885
AFFX-CreX-5_at -0.04016781
AFFX-CreX-3_at -0.026889801
AFFX-DapX-5_at 0.08538008
AFFX-DapX-M_at 0.20443916
AFFX-DapX-3_at 0.23267365
AFFX-LysX-5_at 0.16799259
AFFX-LysX-M_at -0.06620026
AFFX-LysX-3_at 0.1666069
AFFX-PheX-5_at 0.15115786
AFFX-PheX-M_at 0.1564331
AFFX-PheX-3_at 0.023828506
AFFX-ThrX-5_at -0.12991714
AFFX-ThrX-M_at 0.12447405

Total number of rows: 45101

Table truncated, full table size 1015 Kbytes.

Supplementary file Size Download File type/resource
GSM2386196_RSM17739.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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