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Sample GSM2386195 Query DataSets for GSM2386195
Status Public on Aug 01, 2017
Title Batf+/+ Treg_3
Sample type RNA
Source name Batf+/+ Treg cells
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: lymph node and spleen
cell type: hCD2(+)CD4(+) cells
sorted from: FoxP3hCD2.Batf+/+ mice
Treatment protocol None
Growth protocol ex vivo isolated cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
Label biotin
Label protocol RNA was subjected to biotinylated cDNA synthesis by using GeneChip 3'IVT Express Kit.
Hybridization protocol The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description freshly sorted from FoxP3hCD2.Batf+/+ mice
Data processing Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
Submission date Nov 08, 2016
Last update date Aug 01, 2017
Contact name Shohei Hori
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platform ID GPL1261
Series (2)
GSE89656 Expression data from BATF-deficient Treg cells
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
AFFX-BioB-5_at 0.53183556
AFFX-BioB-M_at 0.61930084
AFFX-BioB-3_at 0.55733967
AFFX-BioC-5_at 0.48764324
AFFX-BioC-3_at 0.50085735
AFFX-BioDn-5_at 0.50485516
AFFX-BioDn-3_at 0.4253683
AFFX-CreX-5_at 0.21385193
AFFX-CreX-3_at 0.2107687
AFFX-DapX-5_at -0.5701294
AFFX-DapX-M_at -0.41393661
AFFX-DapX-3_at -0.3270445
AFFX-LysX-5_at -0.20964003
AFFX-LysX-M_at -0.3774824
AFFX-LysX-3_at -0.343987
AFFX-PheX-5_at -0.18850803
AFFX-PheX-M_at -0.31689215
AFFX-PheX-3_at -0.13216877
AFFX-ThrX-5_at -0.19269276
AFFX-ThrX-M_at -0.40680027

Total number of rows: 45101

Table truncated, full table size 889 Kbytes.

Supplementary file Size Download File type/resource
GSM2386195_RSM18102.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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