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Sample GSM2386193 Query DataSets for GSM2386193
Status Public on Aug 01, 2017
Title Batf+/+ Treg_1
Sample type RNA
 
Source name Batf+/+ Treg cells
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: lymph node and spleen
cell type: hCD2(+)CD4(+) cells
sorted from: FoxP3hCD2.Batf+/+ mice
Treatment protocol None
Growth protocol ex vivo isolated cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
Label biotin
Label protocol RNA was subjected to biotinylated cDNA synthesis by using GeneChip 3'IVT Express Kit.
 
Hybridization protocol The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description freshly sorted from FoxP3hCD2.Batf+/+ mice
Data processing Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
 
Submission date Nov 08, 2016
Last update date Aug 01, 2017
Contact name Shohei Hori
E-mail(s) shohei.hori@riken.jp
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
 
Platform ID GPL1261
Series (2)
GSE89656 Expression data from BATF-deficient Treg cells
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at -0.18522644
AFFX-BioB-M_at -0.21015644
AFFX-BioB-3_at -0.19619846
AFFX-BioC-5_at -0.23640823
AFFX-BioC-3_at -0.23704815
AFFX-BioDn-5_at -0.16553116
AFFX-BioDn-3_at -0.20517445
AFFX-CreX-5_at -0.10663605
AFFX-CreX-3_at -0.14278889
AFFX-DapX-5_at 0
AFFX-DapX-M_at 0
AFFX-DapX-3_at 0
AFFX-LysX-5_at 0
AFFX-LysX-M_at 0
AFFX-LysX-3_at 0
AFFX-PheX-5_at 0
AFFX-PheX-M_at 0
AFFX-PheX-3_at 0
AFFX-ThrX-5_at 0.1640544
AFFX-ThrX-M_at 0

Total number of rows: 45101

Table truncated, full table size 889 Kbytes.




Supplementary file Size Download File type/resource
GSM2386193_RSM17737.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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