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Sample GSM2386176 Query DataSets for GSM2386176
Status Public on Aug 01, 2017
Title R397W Treg_3
Sample type RNA
Source name hCD2(+)GFP(-)CD4(+) cells sorted from FoxP3R397W:hCD2/WT:GFP mice
Organism Mus musculus
Characteristics strain: B6;129
tissue: lymph nodes and spleen
cell type: hCD2(+)GFP(-)CD4(+) cells
sorted from: FoxP3R397W:hCD2/WT:GFP mice
Treatment protocol None
Growth protocol ex vivo isolated cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
Label biotin
Label protocol RNA was subjected to biotinylated cDNA synthesis by using GeneChip Two-Cycle Target Labeling and Control Reagents.
Hybridization protocol The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description freshly isolated cells
Data processing Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
Submission date Nov 08, 2016
Last update date Aug 01, 2017
Contact name Shohei Hori
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platform ID GPL1261
Series (2)
GSE89654 Expression data from Treg cells expressing mutant FoxP3
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
AFFX-BioB-5_at -0.51560116
AFFX-BioB-M_at -0.72590923
AFFX-BioB-3_at -0.5850811
AFFX-BioC-5_at -0.43653965
AFFX-BioC-3_at -0.48786354
AFFX-BioDn-5_at -0.15613556
AFFX-BioDn-3_at -0.19753456
AFFX-CreX-5_at -0.10260296
AFFX-CreX-3_at -0.13838482
AFFX-DapX-5_at -1.6327066
AFFX-DapX-M_at -1.0017176
AFFX-DapX-3_at -1.1804056
AFFX-LysX-5_at -0.9716854
AFFX-LysX-M_at -1.5715284
AFFX-LysX-3_at -1.450654
AFFX-PheX-5_at -1.252706
AFFX-PheX-M_at -1.0886335
AFFX-PheX-3_at -1.183157
AFFX-ThrX-5_at -1.3030305
AFFX-ThrX-M_at -1.611536

Total number of rows: 45101

Table truncated, full table size 1004 Kbytes.

Supplementary file Size Download File type/resource
GSM2386176_RSM00937.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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