GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM2386175 Query DataSets for GSM2386175
Status Public on Aug 01, 2017
Title R397W Treg_2
Sample type RNA
Source name hCD2(+)GFP(-)CD4(+) cells sorted from FoxP3R397W:hCD2/WT:GFP mice
Organism Mus musculus
Characteristics strain: B6;129
tissue: lymph nodes and spleen
cell type: hCD2(+)GFP(-)CD4(+) cells
sorted from: FoxP3R397W:hCD2/WT:GFP mice
Treatment protocol None
Growth protocol ex vivo isolated cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
Label biotin
Label protocol RNA was subjected to biotinylated cDNA synthesis by using GeneChip Two-Cycle Target Labeling and Control Reagents.
Hybridization protocol The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description freshly isolated cells
Data processing Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
Submission date Nov 08, 2016
Last update date Aug 01, 2017
Contact name Shohei Hori
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platform ID GPL1261
Series (2)
GSE89654 Expression data from Treg cells expressing mutant FoxP3
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
AFFX-BioB-5_at -0.03728962
AFFX-BioB-M_at -0.04449272
AFFX-BioB-3_at -0.07188797
AFFX-BioC-5_at -0.010533333
AFFX-BioC-3_at -0.019235611
AFFX-BioDn-5_at 0.36356926
AFFX-BioDn-3_at 0.15671825
AFFX-CreX-5_at 0.27148247
AFFX-CreX-3_at 0.22751331
AFFX-DapX-5_at -0.5689926
AFFX-DapX-M_at -0.7797003
AFFX-DapX-3_at -0.73568726
AFFX-LysX-5_at -1.3333263
AFFX-LysX-M_at -1.3257923
AFFX-LysX-3_at -0.9049444
AFFX-PheX-5_at -0.85616636
AFFX-PheX-M_at -1.1622229
AFFX-PheX-3_at -0.901824
AFFX-ThrX-5_at -0.8918557
AFFX-ThrX-M_at -1.0564613

Total number of rows: 45101

Table truncated, full table size 1010 Kbytes.

Supplementary file Size Download File type/resource
GSM2386175_RSM00661.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap