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Sample GSM2386173 Query DataSets for GSM2386173
Status Public on Aug 01, 2017
Title I363V Treg_3
Sample type RNA
Source name hCD2(+)GFP(-)CD4(+) cells sorted from FoxP3I363V:hCD2/WT:GFP mice
Organism Mus musculus
Characteristics strain: B6;129
tissue: lymph nodes and spleen
cell type: hCD2(+)GFP(-)CD4(+) cells
sorted from: FoxP3I363V:hCD2/WT:GFP mice
Treatment protocol None
Growth protocol ex vivo isolated cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
Label biotin
Label protocol RNA was subjected to biotinylated cDNA synthesis by using GeneChip Two-Cycle Target Labeling and Control Reagents.
Hybridization protocol The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description freshly isolated cells
Data processing Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
Submission date Nov 08, 2016
Last update date Aug 01, 2017
Contact name Shohei Hori
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platform ID GPL1261
Series (2)
GSE89654 Expression data from Treg cells expressing mutant FoxP3
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
AFFX-BioB-5_at -0.15182114
AFFX-BioB-M_at -0.047941208
AFFX-BioB-3_at -0.09252262
AFFX-BioC-5_at -0.0992403
AFFX-BioC-3_at -0.09708309
AFFX-BioDn-5_at 0.21387386
AFFX-BioDn-3_at 0.003533363
AFFX-CreX-5_at 0.15197372
AFFX-CreX-3_at 0.12187672
AFFX-DapX-5_at -0.7949343
AFFX-DapX-M_at -0.79194546
AFFX-DapX-3_at -1.086832
AFFX-LysX-5_at -0.602942
AFFX-LysX-M_at -1.0267744
AFFX-LysX-3_at -1.1302309
AFFX-PheX-5_at -1.04532
AFFX-PheX-M_at -1.0949898
AFFX-PheX-3_at -1.2634287
AFFX-ThrX-5_at -1.2265859
AFFX-ThrX-M_at -1.3846903

Total number of rows: 45101

Table truncated, full table size 1011 Kbytes.

Supplementary file Size Download File type/resource
GSM2386173_RSM00592.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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