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Sample GSM2386170 Query DataSets for GSM2386170
Status Public on Aug 01, 2017
Title A384T Treg_3
Sample type RNA
 
Source name hCD2(+)GFP(-)CD4(+) cells sorted from FoxP3A384T:hCD2/WT:GFP mice
Organism Mus musculus
Characteristics strain: B6;129
tissue: lymph nodes and spleen
cell type: hCD2(+)GFP(-)CD4(+) cells
sorted from: FoxP3A384T:hCD2/WT:GFP mice
Treatment protocol None
Growth protocol ex vivo isolated cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
Label biotin
Label protocol RNA was subjected to biotinylated cDNA synthesis by using GeneChip Two-Cycle Target Labeling and Control Reagents.
 
Hybridization protocol The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description freshly isolated cells
Data processing Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
 
Submission date Nov 08, 2016
Last update date Aug 01, 2017
Contact name Shohei Hori
E-mail(s) shohei.hori@riken.jp
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
 
Platform ID GPL1261
Series (2)
GSE89654 Expression data from Treg cells expressing mutant FoxP3
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at -0.670208
AFFX-BioB-M_at -0.4807682
AFFX-BioB-3_at -0.55494595
AFFX-BioC-5_at -0.5208025
AFFX-BioC-3_at -0.48148918
AFFX-BioDn-5_at -0.1682005
AFFX-BioDn-3_at -0.2676792
AFFX-CreX-5_at 0.06947422
AFFX-CreX-3_at 0.05779457
AFFX-DapX-5_at 0.4947586
AFFX-DapX-M_at 0.06859875
AFFX-DapX-3_at -0.38743687
AFFX-LysX-5_at -0.17033052
AFFX-LysX-M_at -0.41654015
AFFX-LysX-3_at -0.5432987
AFFX-PheX-5_at -0.34356737
AFFX-PheX-M_at -0.3826542
AFFX-PheX-3_at -0.6122179
AFFX-ThrX-5_at -0.3301015
AFFX-ThrX-M_at -0.35181522

Total number of rows: 45101

Table truncated, full table size 1009 Kbytes.




Supplementary file Size Download File type/resource
GSM2386170_RSM00460.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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