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Sample GSM2386166 Query DataSets for GSM2386166
Status Public on Aug 01, 2017
Title WT Treg (GFP+ from A384T:hCD2/WT:GFP mice)_2
Sample type RNA
Source name hCD2(-)GFP(+)CD4(+) cells sorted from FoxP3A384T:hCD2/WT:GFP mice
Organism Mus musculus
Characteristics strain: B6;129
tissue: lymph nodes and spleen
cell type: hCD2(-)GFP(+)CD4(+) cells
sorted from: FoxP3A384T:hCD2/WT:GFP mice
Treatment protocol None
Growth protocol ex vivo isolated cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
Label biotin
Label protocol RNA was subjected to biotinylated cDNA synthesis by using GeneChip Two-Cycle Target Labeling and Control Reagents.
Hybridization protocol The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description freshly isolated cells
Data processing Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
Submission date Nov 08, 2016
Last update date Aug 01, 2017
Contact name Shohei Hori
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platform ID GPL1261
Series (2)
GSE89654 Expression data from Treg cells expressing mutant FoxP3
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
AFFX-BioB-5_at -0.8021765
AFFX-BioB-M_at -0.80492973
AFFX-BioB-3_at -0.77474594
AFFX-BioC-5_at -0.7222004
AFFX-BioC-3_at -0.6712599
AFFX-BioDn-5_at -0.7289438
AFFX-BioDn-3_at -0.80529976
AFFX-CreX-5_at -0.71670055
AFFX-CreX-3_at -0.66504097
AFFX-DapX-5_at -1.7252622
AFFX-DapX-M_at -1.7517529
AFFX-DapX-3_at -1.9608669
AFFX-LysX-5_at -1.2108011
AFFX-LysX-M_at -1.764936
AFFX-LysX-3_at -1.7870626
AFFX-PheX-5_at -1.8099151
AFFX-PheX-M_at -2.025569
AFFX-PheX-3_at -2.246725
AFFX-ThrX-5_at -1.678555
AFFX-ThrX-M_at -1.9674883

Total number of rows: 45101

Table truncated, full table size 1007 Kbytes.

Supplementary file Size Download File type/resource
GSM2386166_RSM00190.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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