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Sample GSM2386163 Query DataSets for GSM2386163
Status Public on Aug 01, 2017
Title WT Treg (hCD2+ from WT:hCD2/WT:GFP mice)_3
Sample type RNA
Source name hCD2(+)GFP(-)CD4(+) cells sorted from FoxP3WT:hCD2/WT:GFP mice
Organism Mus musculus
Characteristics strain: B6;129
tissue: lymph nodes and spleen
cell type: hCD2(+)GFP(-)CD4(+) cells
sorted from: FoxP3WT:hCD2/WT:GFP mice
Treatment protocol None
Growth protocol ex vivo isolated cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Isogen reagent (Nippon Gene) followed by RNeasy mini kit (Qiagen).
Label biotin
Label protocol RNA was subjected to biotinylated cDNA synthesis by using GeneChip Two-Cycle Target Labeling and Control Reagents.
Hybridization protocol The labeled cDNA was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 Arrays using Affymetrix standard procedures.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Description freshly isolated cells
Data processing Expression values for each probe set were calculated with rma method in a GeneSpring GX software package (version 13.0) (Agilent).
Submission date Nov 08, 2016
Last update date Aug 01, 2017
Contact name Shohei Hori
Phone +81-45-503-7069
Organization name RIKEN Center for Integrative Medical Sciences
Lab Laboratory for Immune Homeostasis
Street address 1-7-22 Suehiro-cho, Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platform ID GPL1261
Series (2)
GSE89654 Expression data from Treg cells expressing mutant FoxP3
GSE89744 Analyses of a mutant FoxP3 allele reveal BATF as a critical transcription factor in the differentiation and accumulation of tissue regulatory T cells

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
AFFX-BioB-5_at -0.40776443
AFFX-BioB-M_at -0.46238613
AFFX-BioB-3_at -0.380579
AFFX-BioC-5_at -0.31874657
AFFX-BioC-3_at -0.29878902
AFFX-BioDn-5_at -0.3520527
AFFX-BioDn-3_at -0.38964176
AFFX-CreX-5_at -0.2743988
AFFX-CreX-3_at -0.29629993
AFFX-DapX-5_at -0.58511543
AFFX-DapX-M_at -0.65060234
AFFX-DapX-3_at -0.80096245
AFFX-LysX-5_at -0.49900675
AFFX-LysX-M_at -0.6975136
AFFX-LysX-3_at -0.7679186
AFFX-PheX-5_at -0.7871628
AFFX-PheX-M_at -1.0624428
AFFX-PheX-3_at -1.1745787
AFFX-ThrX-5_at -0.73016596
AFFX-ThrX-M_at -0.9583111

Total number of rows: 45101

Table truncated, full table size 1008 Kbytes.

Supplementary file Size Download File type/resource
GSM2386163_RSM00218.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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