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Sample GSM2372584 Query DataSets for GSM2372584
Status Public on Dec 01, 2017
Title Mock_3
Sample type RNA
Source name Human dermal fibroblasts introduced empty vector (Mock), replicate 3
Organism Homo sapiens
Characteristics cell type: Reprogramming cells
Treatment protocol The samples on day3 were EGFP (+) cells collected by flow cytometry. TRA-1-60 (+) cells on days 7-28 were collected by magnetic activated cell sorting.
Growth protocol Somatic cell lines were maintained in appropriate conditions according to manufacturer's recommendation. TRA-1-60 (+) cells and iPSCs were maintained on SNL feeders in Primate ESC medium (ReproCELL) supplemented with 4 ng/ml basic fibroblast growth factor at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the miReasy Purification kit (Qiagen) following the manufacturer's recommendations, and quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Manufature's protocol
Hybridization protocol Manufature's protocol
Scan protocol Slides were scanned on the G2565CA Microarray Scanner System (Agilent).
Description Human dermal fibroblasts introduced empty vector (Mock), replicate 3
Data processing GeneSpring GX. Signals were normalized to percentile.
Submission date Nov 02, 2016
Last update date Dec 01, 2017
Contact name Kazutoshi Takahashi
Organization name Kyoto University
Department Center for iPS Cell Research and Application
Street address 53 Kawahara-cho, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
Platform ID GPL13607
Series (1)
GSE89455 Proliferation pause as an early blockade of human cellular reprogramming toward pluripotency

Data table header descriptions
VALUE Normalized signal intensity

Data table
1 7.5928097
2 -5.2972746
4 -1.8644638
5 1.1284084
6 -3.1940136
7 3.3434238
8 1.2045393
10 -3.1780944
11 -5.2781763
12 -0.61671543
13 -0.03060627
14 -0.6528549
15 3.9742613
16 -5.2689896
17 -2.694283
18 -5.268022
19 -5.337187
20 1.6085272
21 1.2555189
22 -5.3068447

Total number of rows: 42060

Table truncated, full table size 667 Kbytes.

Supplementary file Size Download File type/resource
GSM2372584_Mock_3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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