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Sample GSM2372551 Query DataSets for GSM2372551
Status Public on Dec 01, 2017
Title OS_3
Sample type RNA
Source name Human dermal fibroblasts expressing OS, replicate 3
Organism Homo sapiens
Characteristics cell type: Reprogramming cells
Treatment protocol The samples on day3 were EGFP (+) cells collected by flow cytometry. TRA-1-60 (+) cells on days 7-28 were collected by magnetic activated cell sorting.
Growth protocol Somatic cell lines were maintained in appropriate conditions according to manufacturer's recommendation. TRA-1-60 (+) cells and iPSCs were maintained on SNL feeders in Primate ESC medium (ReproCELL) supplemented with 4 ng/ml basic fibroblast growth factor at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the miReasy Purification kit (Qiagen) following the manufacturer's recommendations, and quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Manufature's protocol
Hybridization protocol Manufature's protocol
Scan protocol Slides were scanned on the G2565CA Microarray Scanner System (Agilent).
Description Human dermal fibroblasts expressing OS, replicate 3
Data processing GeneSpring GX. Signals were normalized to percentile.
Submission date Nov 02, 2016
Last update date Dec 01, 2017
Contact name Kazutoshi Takahashi
Organization name Kyoto University
Department Center for iPS Cell Research and Application
Street address 53 Kawahara-cho, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
Platform ID GPL13607
Series (1)
GSE89455 Proliferation pause as an early blockade of human cellular reprogramming toward pluripotency

Data table header descriptions
VALUE Normalized signal intensity

Data table
1 7.16971
2 -5.6212626
4 -1.0208101
5 0.8318062
6 -1.9345827
7 3.494771
8 0.7174473
10 -2.5538964
11 -3.0433655
12 -0.51646614
13 0.018651009
14 -0.8486848
15 2.877903
16 -3.7604332
17 -2.689424
18 -3.8509884
19 -4.8375444
20 1.1538677
21 1.3563938
22 -5.5750856

Total number of rows: 42060

Table truncated, full table size 667 Kbytes.

Supplementary file Size Download File type/resource
GSM2372551_OS_3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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