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Sample GSM2372500 Query DataSets for GSM2372500
Status Public on Dec 01, 2017
Title HBEC_d3_3
Sample type RNA
 
Source name EGFP (+) cells expressing OSKM on day 3 derived from Human bronchial epithelial cells, replicate3
Organism Homo sapiens
Characteristics cell type: Reprogramming cells
Treatment protocol The samples on day3 were EGFP (+) cells collected by flow cytometry. TRA-1-60 (+) cells on days 7-28 were collected by magnetic activated cell sorting.
Growth protocol Somatic cell lines were maintained in appropriate conditions according to manufacturer's recommendation. TRA-1-60 (+) cells and iPSCs were maintained on SNL feeders in Primate ESC medium (ReproCELL) supplemented with 4 ng/ml basic fibroblast growth factor at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the miReasy Purification kit (Qiagen) following the manufacturer's recommendations, and quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Manufature's protocol
 
Hybridization protocol Manufature's protocol
Scan protocol Slides were scanned on the G2565CA Microarray Scanner System (Agilent).
Description EGFP (+) cells expressing OSKM on day 3 derived from Human bronchial epithelial cells, replicate3
Data processing GeneSpring GX. Signals were normalized to percentile.
 
Submission date Nov 02, 2016
Last update date Dec 01, 2017
Contact name Kazutoshi Takahashi
E-mail(s) kazu@cira.kyoto-u.ac.jp
Organization name Kyoto University
Department Center for iPS Cell Research and Application
Street address 53 Kawahara-cho, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
 
Platform ID GPL13607
Series (1)
GSE89455 Proliferation pause as an early blockade of human cellular reprogramming toward pluripotency

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
1 8.119434
2 -6.252804
4 -0.47141075
5 -2.3591871
6 -3.262124
7 3.9148588
8 1.6448202
10 -4.00709
11 -5.677888
12 1.3706245
13 -0.02674961
14 0.035513878
15 0.49782753
16 -6.403042
17 -1.6722951
18 -6.403474
19 -6.488558
20 1.0619392
21 2.3855333
22 -5.9357805

Total number of rows: 42060

Table truncated, full table size 667 Kbytes.




Supplementary file Size Download File type/resource
GSM2372500_HBEC_d3_3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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