|
Status |
Public on Dec 01, 2017 |
Title |
HA_d28_2 |
Sample type |
RNA |
|
|
Source name |
TRA-1-60 (+) cells expressing OSKM on day 28 derived from Human astrocytes, replicate2
|
Organism |
Homo sapiens |
Characteristics |
cell type: Reprogramming cells
|
Treatment protocol |
The samples on day3 were EGFP (+) cells collected by flow cytometry. TRA-1-60 (+) cells on days 7-28 were collected by magnetic activated cell sorting.
|
Growth protocol |
Somatic cell lines were maintained in appropriate conditions according to manufacturer's recommendation. TRA-1-60 (+) cells and iPSCs were maintained on SNL feeders in Primate ESC medium (ReproCELL) supplemented with 4 ng/ml basic fibroblast growth factor at 37 ÂșC in a humidified incubator with 5% CO2.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was prepared using the miReasy Purification kit (Qiagen) following the manufacturer's recommendations, and quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
|
Label |
Cy3
|
Label protocol |
Manufature's protocol
|
|
|
Hybridization protocol |
Manufature's protocol
|
Scan protocol |
Slides were scanned on the G2565CA Microarray Scanner System (Agilent).
|
Description |
TRA-1-60 (+) cells expressing OSKM on day 28 derived from Human astrocytes, replicate2
|
Data processing |
GeneSpring GX. Signals were normalized to percentile.
|
|
|
Submission date |
Nov 02, 2016 |
Last update date |
Dec 01, 2017 |
Contact name |
Kazutoshi Takahashi |
E-mail(s) |
kazu@cira.kyoto-u.ac.jp
|
Organization name |
Kyoto University
|
Department |
Center for iPS Cell Research and Application
|
Street address |
53 Kawahara-cho, Shogoin, Sakyo-ku
|
City |
Kyoto |
ZIP/Postal code |
606-8507 |
Country |
Japan |
|
|
Platform ID |
GPL13607 |
Series (1) |
GSE89455 |
Proliferation pause as an early blockade of human cellular reprogramming toward pluripotency |
|