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Sample GSM2372482 Query DataSets for GSM2372482
Status Public on Dec 01, 2017
Title HA_d7_3
Sample type RNA
 
Source name TRA-1-60 (+) cells expressing OSKM on day 7 derived from Human astrocytes, replicate3
Organism Homo sapiens
Characteristics cell type: Reprogramming cells
Treatment protocol The samples on day3 were EGFP (+) cells collected by flow cytometry. TRA-1-60 (+) cells on days 7-28 were collected by magnetic activated cell sorting.
Growth protocol Somatic cell lines were maintained in appropriate conditions according to manufacturer's recommendation. TRA-1-60 (+) cells and iPSCs were maintained on SNL feeders in Primate ESC medium (ReproCELL) supplemented with 4 ng/ml basic fibroblast growth factor at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the miReasy Purification kit (Qiagen) following the manufacturer's recommendations, and quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Manufature's protocol
 
Hybridization protocol Manufature's protocol
Scan protocol Slides were scanned on the G2565CA Microarray Scanner System (Agilent).
Description TRA-1-60 (+) cells expressing OSKM on day 7 derived from Human astrocytes, replicate3
Data processing GeneSpring GX. Signals were normalized to percentile.
 
Submission date Nov 02, 2016
Last update date Dec 01, 2017
Contact name Kazutoshi Takahashi
E-mail(s) kazu@cira.kyoto-u.ac.jp
Organization name Kyoto University
Department Center for iPS Cell Research and Application
Street address 53 Kawahara-cho, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
 
Platform ID GPL13607
Series (1)
GSE89455 Proliferation pause as an early blockade of human cellular reprogramming toward pluripotency

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
1 7.610944
2 -6.3915534
4 -1.6175218
5 0.08850193
6 -2.8563962
7 3.1402817
8 1.4617682
10 -3.8222303
11 -6.4224815
12 -0.026057243
13 1.1467476
14 -0.041822433
15 4.543043
16 -6.401323
17 -1.3890862
18 -1.012157
19 1.9203281
20 1.3392248
21 0.48146248
22 -6.0118

Total number of rows: 42060

Table truncated, full table size 668 Kbytes.




Supplementary file Size Download File type/resource
GSM2372482_HA_d7_3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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