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Sample GSM2372461 Query DataSets for GSM2372461
Status Public on Dec 01, 2017
Title HAdMSC_d7_3
Sample type RNA
 
Source name TRA-1-60 (+) cells expressing OSKM on day 7 derived from Human adipose-derived mesenchymal stem cells, replicate3
Organism Homo sapiens
Characteristics cell type: Reprogramming cells
Treatment protocol The samples on day3 were EGFP (+) cells collected by flow cytometry. TRA-1-60 (+) cells on days 7-28 were collected by magnetic activated cell sorting.
Growth protocol Somatic cell lines were maintained in appropriate conditions according to manufacturer's recommendation. TRA-1-60 (+) cells and iPSCs were maintained on SNL feeders in Primate ESC medium (ReproCELL) supplemented with 4 ng/ml basic fibroblast growth factor at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the miReasy Purification kit (Qiagen) following the manufacturer's recommendations, and quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Manufature's protocol
 
Hybridization protocol Manufature's protocol
Scan protocol Slides were scanned on the G2565CA Microarray Scanner System (Agilent).
Description TRA-1-60 (+) cells expressing OSKM on day 7 derived from Human adipose-derived mesenchymal stem cells, replicate3
Data processing GeneSpring GX. Signals were normalized to percentile.
 
Submission date Nov 02, 2016
Last update date Dec 01, 2017
Contact name Kazutoshi Takahashi
E-mail(s) kazu@cira.kyoto-u.ac.jp
Organization name Kyoto University
Department Center for iPS Cell Research and Application
Street address 53 Kawahara-cho, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
 
Platform ID GPL13607
Series (1)
GSE89455 Proliferation pause as an early blockade of human cellular reprogramming toward pluripotency

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
1 7.4989223
2 -6.527362
4 -1.5178151
5 -0.78715515
6 -3.3054943
7 3.5649405
8 1.905344
10 -3.407044
11 -6.5320644
12 0.28613377
13 1.1203232
14 0.99294186
15 2.8968306
16 -6.52717
17 -1.4415588
18 -0.7398262
19 -5.8556566
20 1.1968699
21 0.592289
22 -6.1183014

Total number of rows: 42060

Table truncated, full table size 669 Kbytes.




Supplementary file Size Download File type/resource
GSM2372461_HAdMSC_d7_3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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