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Sample GSM2372432 Query DataSets for GSM2372432
Status Public on Dec 01, 2017
Title HDF_d0_1
Sample type RNA
Source name Human dermal fibroblasts, replicate1
Organism Homo sapiens
Characteristics cell type: Somatic cell line
Treatment protocol The samples on day3 were EGFP (+) cells collected by flow cytometry. TRA-1-60 (+) cells on days 7-28 were collected by magnetic activated cell sorting.
Growth protocol Somatic cell lines were maintained in appropriate conditions according to manufacturer's recommendation. TRA-1-60 (+) cells and iPSCs were maintained on SNL feeders in Primate ESC medium (ReproCELL) supplemented with 4 ng/ml basic fibroblast growth factor at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the miReasy Purification kit (Qiagen) following the manufacturer's recommendations, and quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Manufature's protocol
Hybridization protocol Manufature's protocol
Scan protocol Slides were scanned on the G2565CA Microarray Scanner System (Agilent).
Description Human dermal fibroblasts, replicate1
Data processing GeneSpring GX. Signals were normalized to percentile.
Submission date Nov 02, 2016
Last update date Dec 01, 2017
Contact name Kazutoshi Takahashi
Organization name Kyoto University
Department Center for iPS Cell Research and Application
Street address 53 Kawahara-cho, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
Platform ID GPL13607
Series (1)
GSE89455 Proliferation pause as an early blockade of human cellular reprogramming toward pluripotency

Data table header descriptions
VALUE Normalized signal intensity

Data table
1 7.687683
2 -6.7911506
4 -1.2725964
5 1.1022835
6 -3.015273
7 3.3576126
8 1.1995869
10 -3.412057
11 -6.770136
12 -0.38767433
13 0.7093468
14 -0.6132169
15 4.6058645
16 -6.7634397
17 -1.3935194
18 -6.763734
19 -6.3815727
20 1.4287615
21 1.4618511
22 -6.5143213

Total number of rows: 42060

Table truncated, full table size 667 Kbytes.

Supplementary file Size Download File type/resource
GSM2372432_HDF_d0_1.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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