|
| Status |
Public on Sep 25, 2017 |
| Title |
G1789_G2KO_B_IL7R |
| Sample type |
SRA |
| |
|
| Source name |
bone marrow cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype: PLCgamma1+/+PLCgamma2-/-
cell population: B cell progenitor
facs sorting surface marker: B220+IgM-IL-7R+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by using TRIzol LS reagent. mRNA was purified by using NEBNext poly(A) mRNA magnetic Isolation module.
Library was prepared by using NEBNext Ultra RNA Library Prep Kit for Illumina.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
B_IL7R PLC Gamma2 KO replicate 2
|
| Data processing |
RNA-seq alignment was done by RNA-Seq Alignment software v1.0, Illumina, Inc. Sequences were un-trimmed and aligned to Mus musculus UCSC mm10 RefSeq by STAR aligner v2.5.0a.
Genome_build: mm10
Supplementary_files_format_and_content: Gene counts as output from STAR aligner
|
| |
|
| Submission date |
Oct 31, 2016 |
| Last update date |
Sep 26, 2017 |
| Contact name |
Geoffrey Neale |
| E-mail(s) |
geoffrey.neale@stjude.org
|
| Organization name |
St Jude Childrens Research Hospital
|
| Department |
Hartwell Center
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE89352 |
Phospholipase Cγ pathway-dependent mTOR signaling controls IL-7-mediated early B cell development |
|
| Relations |
| BioSample |
SAMN05956357 |
