|Public on Jan 12, 2017
|cell type: Presentation ALL patient sample engrafred in mice and harvested from spleen
|SEM cells were continuously grown in IMDM with 10-20% FBS, split when they reached a density of 1-2X10e6 down to 5X10e5
|ChIP-seq: Fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated (~200bp) and protein-DNA complexes were isolated with antibody.
Libraries were prepared according to Illumina's protocol accompanying the DNA Library Prep Master Mix Set (Part# E6040).
|Illumina NextSeq 500
|ChIP-seq reads were aligned to the hg18 genome using bowtie version 1.1.2 with following parameters: p1 m2 best strata sam
Peaks were called using SeqMonk version 0.24.1
Supplementary_files_format_and_content: bed files were generated from text files outputted directly from SeqMonk
|Oct 28, 2016
|Last update date
|May 15, 2019
|Weatherall Institute of Molecular Medicine
|Molecular Haematology Unit
|John Radcliffe Hospital
|MLL-AF4 Spreading Identifies Binding Sites that Are Distinct from Super-Enhancers and that Govern Sensitivity to DOT1L Inhibition in Leukemia.