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Sample GSM2363514 Query DataSets for GSM2363514
Status Public on Feb 16, 2017
Title Calorie Restricted (22 month) Rep 1
Sample type SRA
Source name Hepatocytes
Organism Mus musculus
Characteristics sample type: UM-HET3
treatment: Calorie Restriction Treated
Treatment protocol Some mice were given encapsulated rapamycin at a drug dose of 42 ppm from 4 months of age. For other mice, a calorie restricted diet was initiated at 4 months; these mice received 60% of the food consumed by age-matched controls, after a two week run-in period at 80%.
Growth protocol Genetically hetrogenous UM-HET3 female mice were bred and housed at the university of Michigan. They are the offspring of (BALB/cByJ x C57BL/6J)F1 mothers and (C3H/HeJ x DBA/2J)F1 fathers. They were housed in a specific-pathogen free colony at four mice/cage and given a diet based on Purina 5LG6.
Extracted molecule genomic DNA
Extraction protocol DNA was extracted by Dneasy blood and tissue kit (Quiagen)
Libraries were prepared by BGI, Shenzhen. Briefly, DNA is fragmented and methylated adapters are ligated to DNA fragments. DNA is bisulphite treated and sequenced on Illumina platforms as per manufacturers instructions.
Library strategy Bisulfite-Seq
Library source genomic
Library selection RANDOM
Instrument model Illumina HiSeq 4000
Description Bisulfite sequencing of old (22 month) calorie restriction treated mice (UM-HET3)
Data processing Basecalls performed in phred64 (solexa1.3-quals).
Sequenced reads were mapped to mm9 whole genome using bismark v0.10.1 (bowtie2 v2.1.0) with directional setting and chunkmbs set to 256. Remaining default settings.
Multiple reads where both ends of the fragment align to the same genomic positions on the same strand were reduced to a single instance. Reads with more than 3 methylated cytosines in non-CpG contexts are discarded.
Reads are summarised on a per-CpG basis.
Genome_build: mm9
Supplementary_files_format_and_content: Summary files represent the number of reads supporting methylated/unmethylated bases at each CpG sequenced in that sample (Format is: chr <tab> position <tab> methylated reads <tab> unmethylated reads).
Submission date Oct 28, 2016
Last update date May 15, 2019
Contact name Neil A Robertson
Organization name University of Glasgow
Department Beatson Institute for Cancer Research
Lab Kristina Kirschner
Street address Switchback Road
City Glasgow
State/province Scotland
ZIP/Postal code G61 1BD
Country United Kingdom
Platform ID GPL21103
Series (2)
GSE89274 Diverse interventions that extend mouse lifespan suppress shared age-associated epigenetic changes at critical gene regulatory regions (WGBS 2)
GSE89275 Diverse interventions that extend mouse lifespan suppress shared age-associated epigenetic changes at critical gene regulatory regions
Reanalyzed by GSE120136
BioSample SAMN05950873
SRA SRX2279753

Supplementary file Size Download File type/resource
GSM2363514_Cole.CB16.CR.Rep1.CpG_context_aggregate.txt.gz 105.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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