|
| Status |
Public on Dec 30, 2016 |
| Title |
IL10RA wildtype |
| Sample type |
RNA |
| |
|
| Source name |
IL10RA wildtype Tr1-cells isolated from small intestine
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: wildtype
cell type: Tr1-cells
|
| Treatment protocol |
Mice were injected with anti-CD3 (clone 2C11, 15μg,) i.p. two times every other day, and sacrificed 4 hours or 48 hours after the second injection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from cells was isolated with TRIzol LS reagent (Life Technologies) in accordance with the manufacturer’s protocol. RNA was subjected to reverse transcription with Superscript II (Invitrogen) with oligo (dT) primer in accordance with the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled according to standard Affymetrix protocols.
|
| |
|
| Hybridization protocol |
Hybridised to Affymetrix Mouse Gene 1.0 ST Arrays according to standard Affymetrix protocols.
|
| Scan protocol |
Scanned according to standard Affymetrix protocols.
|
| Data processing |
Data was analyzed using R and the Bioconductor package affy. Samples were normalized by RMA with standard parameters.
|
| |
|
| Submission date |
Oct 24, 2016 |
| Last update date |
Dec 30, 2016 |
| Contact name |
Babett Steglich |
| Organization name |
Universitätsklinikum Hamburg-Eppendorf
|
| Street address |
Martinistrasse 52
|
| City |
Hamburg |
| ZIP/Postal code |
20246 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE89080 |
IL-10 Receptor Signaling is essental for Tr1 cell function in vivo |
|
