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Sample GSM2357782 Query DataSets for GSM2357782
Status Public on Oct 22, 2016
Title 121002-lung carcinoma tissue
Sample type RNA
 
Source name lung carcinoma tissue from 121002
Organism Homo sapiens
Characteristics disease status: lung cancer
tissue: lung carcinoma tissue
gender: female
geographic location: Xuanwei County, China
patient id: 121002
Extracted molecule total RNA
Extraction protocol RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
Label Cy3
Label protocol cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine's linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
 
Hybridization protocol DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized in an Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2x SSC at 42℃ for 5 min, and 0.2x SSC for 5 min at room temperature).
Scan protocol Scanned on an Agilent G2565CA scanner.
Description Gene expression of 121002-lung carcinoma tissue.
Data processing Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and GeneSpring software was used to analyze the raw data, which are normalized by percentile normalization.
 
Submission date Oct 21, 2016
Last update date Apr 23, 2018
Contact name Yanliang Zhang
E-mail(s) zylyh2007@163.com
Organization name First Affiliated Hospital of Kunming Medical University
Department Department of Clinical Laboratory
Lab Yunnan Key Laboratory of Laboratory Medicine
Street address No. 295, Xichang Road
City Kunming
State/province Yunnan province
ZIP/Postal code 650032
Country China
 
Platform ID GPL17077
Series (2)
GSE89039 Gene expression profiling in 8 lung cancer in Xuanwei samples
GSE89047 Gene expression profiling and copy number alterations in 8 lung cancer in Xuanwei samples
Relations
Reanalyzed by GSE113533

Data table header descriptions
ID_REF
VALUE Normalized value of 75 percentile (log2)

Data table
ID_REF VALUE
A_23_P117082 3.1330585
A_33_P3246448 -2.7515745
A_33_P3318220 -8.836036
A_33_P3236322 -1.4781742
A_33_P3319925 -8.166928
A_21_P0000509 3.3210402
A_21_P0000744 -0.62049675
A_24_P215804 0.63327694
A_23_P110167 2.757802
A_33_P3211513 0.6508179
A_23_P103349 -6.3250484
A_32_P61480 -6.0548816
A_33_P3788124 -8.906899
A_33_P3414202 -0.8351469
A_33_P3316686 -1.3583508
A_33_P3300975 -1.9567451
A_33_P3263061 2.0989475
A_33_P3261373 -6.7574244
A_24_P278460 -0.5684471
A_21_P0013109 -8.94946

Total number of rows: 50737

Table truncated, full table size 1187 Kbytes.




Supplementary file Size Download File type/resource
GSM2357782_529609A03-A.txt.gz 12.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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