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Sample GSM2357772 Query DataSets for GSM2357772
Status Public on Oct 22, 2016
Title 110901_lung carcinoma tissue
Sample type RNA
Source name lung carcinoma tissue from 110901
Organism Homo sapiens
Characteristics disease status: lung cancer
tissue: lung carcinoma tissue
gender: female
geographic location: Xuanwei County, China
patient id: 110901
Extracted molecule total RNA
Extraction protocol RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
Label Cy3
Label protocol cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine's linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
Hybridization protocol DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized in an Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2x SSC at 42℃ for 5 min, and 0.2x SSC for 5 min at room temperature).
Scan protocol Scanned on an Agilent G2565CA scanner.
Description Gene expression of 110901_lung carcinoma tissue.
Data processing Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and GeneSpring software was used to analyze the raw data, which are normalized by percentile normalization.
Submission date Oct 21, 2016
Last update date Apr 23, 2018
Contact name Yanliang Zhang
Organization name First Affiliated Hospital of Kunming Medical University
Department Department of Clinical Laboratory
Lab Yunnan Key Laboratory of Laboratory Medicine
Street address No. 295, Xichang Road
City Kunming
State/province Yunnan province
ZIP/Postal code 650032
Country China
Platform ID GPL17077
Series (2)
GSE89039 Gene expression profiling in 8 lung cancer in Xuanwei samples
GSE89047 Gene expression profiling and copy number alterations in 8 lung cancer in Xuanwei samples
Reanalyzed by GSE113533

Data table header descriptions
VALUE Normalized value of 75 percentile (log2)

Data table
A_23_P117082 2.1913633
A_33_P3246448 1.5841045
A_33_P3318220 -6.6683817
A_33_P3236322 -0.40281963
A_33_P3319925 -6.165478
A_21_P0000509 -0.29287815
A_21_P0000744 -0.47660923
A_24_P215804 -0.017608643
A_23_P110167 2.6056252
A_33_P3211513 0.25300407
A_23_P103349 -5.6389184
A_32_P61480 -5.7525487
A_33_P3788124 -6.7365584
A_33_P3414202 -1.8029041
A_33_P3316686 -2.2326565
A_33_P3300975 -0.005249977
A_33_P3263061 1.8309145
A_33_P3261373 -5.6211224
A_24_P278460 -0.8596115
A_21_P0013109 -6.7699842

Total number of rows: 50737

Table truncated, full table size 1191 Kbytes.

Supplementary file Size Download File type/resource
GSM2357772_529564A02-A.txt.gz 12.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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