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Sample GSM2357063 Query DataSets for GSM2357063
Status Public on Jan 06, 2017
Title Nat1-null_ESC1_ 2i+LIF
Sample type RNA
 
Source name Nat1-null mouse embryonic stem cells, 2i+LIF
Organism Mus musculus
Characteristics genotype/variation: Nat1-null
cell type: mouse embryonic stem cell line
treatment: 2i+LIF
Treatment protocol Mouse embryonic stem cells were treated with LIF or 2i+LIF.
Growth protocol Standard mouse ESC medium on gelatin-coated plates at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the miRNeasy Purification kit (Qiagen) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Manufacturer's protocol.
 
Hybridization protocol Manufacturer's protocol.
Scan protocol Slides were scanned on the G2565BA Microarray Scanner System (Agilent).
Description Nat1-null mouse embryonic stem cells cultured with 2i+LIF, replicate 1.
Data processing GeneSpring GX. Signals were normalized to percentile.
 
Submission date Oct 20, 2016
Last update date Jan 06, 2017
Contact name Shinya Yamanaka
E-mail(s) yamanaka@cira.kyoto-u.ac.jp
Phone 81-75-366-7041
Organization name Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
Street address 53 Kawahara-machi, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
 
Platform ID GPL10787
Series (2)
GSE89007 Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells [expression]
GSE89012 Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 5.3000927
DarkCorner -5.9642963
A_55_P2051983 -5.995181
A_52_P169082 -1.4168673
A_30_P01028193 -5.9821806
A_52_P237997 -4.3990793
A_51_P414243 1.6853046
A_55_P2136348 -5.967478
A_51_P108228 -5.940708
A_30_P01033363 -4.5087137
A_55_P2049737 -5.956632
A_30_P01024440 -0.5545778
A_30_P01025554 3.5373402
A_30_P01031558 -4.253784
A_30_P01030675 -5.9494114
A_51_P328014 3.6234732
A_30_P01019108 -0.5537977
A_55_P2056220 2.1031952
A_55_P1985764 7.48337
A_52_P108321 -0.22135067

Total number of rows: 55821

Table truncated, full table size 1333 Kbytes.




Supplementary file Size Download File type/resource
GSM2357063_10._NAT1_KO_2i_1.txt.gz 3.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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