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Sample GSM2357062 Query DataSets for GSM2357062
Status Public on Jan 06, 2017
Title Nat1-null_ESC3_LIF
Sample type RNA
Source name Nat1-null mouse embryonic stem cells, LIF
Organism Mus musculus
Characteristics genotype/variation: Nat1-null
cell type: mouse embryonic stem cell line
treatment: LIF
Treatment protocol Mouse embryonic stem cells were treated with LIF or 2i+LIF.
Growth protocol Standard mouse ESC medium on gelatin-coated plates at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the miRNeasy Purification kit (Qiagen) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Manufacturer's protocol.
Hybridization protocol Manufacturer's protocol.
Scan protocol Slides were scanned on the G2565BA Microarray Scanner System (Agilent).
Description Nat1-null mouse embryonic stem cells cultured with LIF, replicate 3.
Data processing GeneSpring GX. Signals were normalized to percentile.
Submission date Oct 20, 2016
Last update date Jan 06, 2017
Contact name Shinya Yamanaka
Phone 81-75-366-7041
Organization name Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
Street address 53 Kawahara-machi, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
Platform ID GPL10787
Series (2)
GSE89007 Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells [expression]
GSE89012 Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 5.085162
DarkCorner -6.2961836
A_55_P2051983 -6.2067556
A_52_P169082 -2.5606365
A_30_P01028193 -6.2956476
A_52_P237997 -6.2900805
A_51_P414243 1.9834156
A_55_P2136348 -6.2814837
A_51_P108228 -5.9015794
A_30_P01033363 -5.600448
A_55_P2049737 -6.2719603
A_30_P01024440 -0.83002853
A_30_P01025554 3.7096977
A_30_P01031558 -5.519259
A_30_P01030675 -6.266052
A_51_P328014 3.8478851
A_30_P01019108 -0.6936827
A_55_P2056220 2.0564232
A_55_P1985764 7.323842
A_52_P108321 -0.96290874

Total number of rows: 55821

Table truncated, full table size 1333 Kbytes.

Supplementary file Size Download File type/resource
GSM2357062_9._NAT1_KO_3.txt.gz 3.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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