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Sample GSM2357061 Query DataSets for GSM2357061
Status Public on Jan 06, 2017
Title Nat1-null_ESC2_LIF
Sample type RNA
 
Source name Nat1-null mouse embryonic stem cells, LIF
Organism Mus musculus
Characteristics genotype/variation: Nat1-null
cell type: mouse embryonic stem cell line
treatment: LIF
Treatment protocol Mouse embryonic stem cells were treated with LIF or 2i+LIF.
Growth protocol Standard mouse ESC medium on gelatin-coated plates at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the miRNeasy Purification kit (Qiagen) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Manufacturer's protocol.
 
Hybridization protocol Manufacturer's protocol.
Scan protocol Slides were scanned on the G2565BA Microarray Scanner System (Agilent).
Description Nat1-null mouse embryonic stem cells cultured with LIF, replicate 2.
Data processing GeneSpring GX. Signals were normalized to percentile.
 
Submission date Oct 20, 2016
Last update date Jan 06, 2017
Contact name Shinya Yamanaka
E-mail(s) yamanaka@cira.kyoto-u.ac.jp
Phone 81-75-366-7041
Organization name Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
Street address 53 Kawahara-machi, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
 
Platform ID GPL10787
Series (2)
GSE89007 Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells [expression]
GSE89012 Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 4.945796
DarkCorner -7.307065
A_55_P2051983 -7.2522154
A_52_P169082 -1.9828181
A_30_P01028193 -5.52498
A_52_P237997 -7.232542
A_51_P414243 2.0834808
A_55_P2136348 -7.2219563
A_51_P108228 -5.0325794
A_30_P01033363 -4.3752575
A_55_P2049737 -7.210084
A_30_P01024440 -0.6970787
A_30_P01025554 3.5098705
A_30_P01031558 -4.4332824
A_30_P01030675 -7.201148
A_51_P328014 3.5489454
A_30_P01019108 -0.82071686
A_55_P2056220 2.0372524
A_55_P1985764 7.259987
A_52_P108321 -1.2075624

Total number of rows: 55821

Table truncated, full table size 1333 Kbytes.




Supplementary file Size Download File type/resource
GSM2357061_8._NAT1_KO_2.txt.gz 3.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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