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Sample GSM2357059 Query DataSets for GSM2357059
Status Public on Jan 06, 2017
Title WT_ESC3_ 2i+LIF
Sample type RNA
Source name wild-type mouse embryonic stem cells, 2i+LIF
Organism Mus musculus
Characteristics genotype/variation: wild-type
cell type: mouse embryonic stem cell line
treatment: 2i+LIF
Treatment protocol Mouse embryonic stem cells were treated with LIF or 2i+LIF.
Growth protocol Standard mouse ESC medium on gelatin-coated plates at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the miRNeasy Purification kit (Qiagen) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Manufacturer's protocol.
Hybridization protocol Manufacturer's protocol.
Scan protocol Slides were scanned on the G2565BA Microarray Scanner System (Agilent).
Description Wild-type mouse embryonic stem cells cultured with 2i+LIF, replicate 3.
Data processing GeneSpring GX. Signals were normalized to percentile.
Submission date Oct 20, 2016
Last update date Jan 06, 2017
Contact name Shinya Yamanaka
Phone 81-75-366-7041
Organization name Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
Street address 53 Kawahara-machi, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
Platform ID GPL10787
Series (2)
GSE89007 Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells [expression]
GSE89012 Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells

Data table header descriptions
VALUE Normalized signal intensity

Data table
GE_BrightCorner 5.0902357
DarkCorner -6.800791
A_55_P2051983 -6.7874675
A_52_P169082 -1.9482689
A_30_P01028193 -6.7659445
A_52_P237997 -6.7557015
A_51_P414243 1.8530998
A_55_P2136348 -6.738516
A_51_P108228 -3.9920607
A_30_P01033363 -4.1454167
A_55_P2049737 -6.7175674
A_30_P01024440 -0.31727695
A_30_P01025554 3.1882954
A_30_P01031558 -4.5685267
A_30_P01030675 -6.6979675
A_51_P328014 3.6140022
A_30_P01019108 -0.6872654
A_55_P2056220 2.3862343
A_55_P1985764 7.683651
A_52_P108321 -1.379283

Total number of rows: 55821

Table truncated, full table size 1333 Kbytes.

Supplementary file Size Download File type/resource
GSM2357059_6._RF8_2i_3.txt.gz 3.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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