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Sample GSM2357058 Query DataSets for GSM2357058
Status Public on Jan 06, 2017
Title WT_ESC2_ 2i+LIF
Sample type RNA
 
Source name wild-type mouse embryonic stem cells, 2i+LIF
Organism Mus musculus
Characteristics genotype/variation: wild-type
cell type: mouse embryonic stem cell line
treatment: 2i+LIF
Treatment protocol Mouse embryonic stem cells were treated with LIF or 2i+LIF.
Growth protocol Standard mouse ESC medium on gelatin-coated plates at 37 ÂșC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the miRNeasy Purification kit (Qiagen) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol Manufacturer's protocol.
 
Hybridization protocol Manufacturer's protocol.
Scan protocol Slides were scanned on the G2565BA Microarray Scanner System (Agilent).
Description Wild-type mouse embryonic stem cells cultured with 2i+LIF, replicate 2.
Data processing GeneSpring GX. Signals were normalized to percentile.
 
Submission date Oct 20, 2016
Last update date Jan 06, 2017
Contact name Shinya Yamanaka
E-mail(s) yamanaka@cira.kyoto-u.ac.jp
Phone 81-75-366-7041
Organization name Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
Street address 53 Kawahara-machi, Shogoin, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
 
Platform ID GPL10787
Series (2)
GSE89007 Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells [expression]
GSE89012 Nat1 promotes translation of specific proteins that induce differentiation of mouse embryonic stem cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 5.1031914
DarkCorner -7.242891
A_55_P2051983 -7.2657933
A_52_P169082 -2.0098615
A_30_P01028193 -6.023931
A_52_P237997 -7.2459
A_51_P414243 1.8736858
A_55_P2136348 -7.234969
A_51_P108228 -4.0755763
A_30_P01033363 -4.18367
A_55_P2049737 -7.221348
A_30_P01024440 -0.3902359
A_30_P01025554 3.279644
A_30_P01031558 -5.2086844
A_30_P01030675 -7.2083683
A_51_P328014 3.654069
A_30_P01019108 -0.9633827
A_55_P2056220 2.4251556
A_55_P1985764 7.672165
A_52_P108321 -1.3812971

Total number of rows: 55821

Table truncated, full table size 1332 Kbytes.




Supplementary file Size Download File type/resource
GSM2357058_5._RF8_2i_2.txt.gz 3.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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