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Sample GSM2345037 Query DataSets for GSM2345037
Status Public on Jul 13, 2017
Title MV4;11_JQ1 click-seq
Sample type SRA
Source name MV4;11 cell line
Organism Homo sapiens
Characteristics cell line: MV4;11
tissue source: peripheral blood
clickable molecule: JQ1
Growth protocol Cell lines were grown in RPMI-1640 supplemented with 20% fetal calf serum, penicillin (100 units/mL) and streptomycin (100 ug/mL)
Extracted molecule genomic DNA
Extraction protocol Lysate were isolated from formaldehybe cross-linked cells and sonicated to release the chromatin, then chem-precipitation was performed with pre-clicked JQ1 biotin conjugated beads
ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics)
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
Data processing Illumina Casava1.8.2 software was used for basecalling.
Reads were aligned to the human genome (G1k V37) using bwa 0.6.2-r126
Peak-calling was performed using MACS2
Genome_build: hg19
Supplementary_files_format_and_content: bed files from MACS2 for each sample
Submission date Oct 14, 2016
Last update date May 15, 2019
Contact name Mark Dawson
Organization name Peter MacCallum Cancer Centre
Street address 305 Grattan Street
City Melbourne
State/province VIC
ZIP/Postal code 3000
Country Australia
Platform ID GPL11154
Series (2)
GSE88748 Click chemistry enables comprehensive preclinical evaluation of targeted epigenetic therapies [Click-Seq 1]
GSE88751 Click chemistry enables comprehensive preclinical evaluation of targeted epigenetic therapies
BioSample SAMN05908452
SRA SRX2245544

Supplementary file Size Download File type/resource
GSM2345037_JQ1_peaks.bed.gz 2.3 Kb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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