GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM2340113 Query DataSets for GSM2340113
Status Public on Oct 09, 2019
Title HEK293T transfected with pNTAP-2×flag empty vector, biological replicate 3
Sample type RNA
Source name Gene expression data from HEK293T transfected with pNTAP-2xflag vector
Organism Homo sapiens
Characteristics cell line: HEK293T
transfection: pNTAP-2×flag empty vector
Treatment protocol Total RNA was isolated from cells 24 hours after transfection using TRIzol reagent (Invitrogen).
Growth protocol The full-length coding sequence of pig VRTN was subcloned into pNTAP-2×flag expression vectors. HEK293T cells were transfected with pNTAP-2×flag-VRTN or empty vectors.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip2 Scanner 3000 7G.
Data processing Netaffx build 36 was used for the analysis. The data were analyzed with Affymetrix Expression Console Software (version 1.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Oct 08, 2016
Last update date Oct 09, 2019
Contact name Yanyu Dean
Phone 86-791-83813080
Organization name Jiangxi Agricultural University
Lab State Key Laboratory of Pig Genetic Improvement and Production Technology
Street address Lushanzhong888, Changbei district
City Nanchang
ZIP/Postal code 330044
Country China
Platform ID GPL6244
Series (2)
GSE87772 Expression data from HEK293T cells transfected with pNTAP-2×flag-VRTN or empty vectors
GSE87776 VRTN Regulates the Number of Thoracic Vertebrae During Vertebrate Development

Data table header descriptions
VALUE log2 GC-RMA signal

Data table
7896738 3.099419
7896740 3.086058
7896742 8.584276
7896744 4.609423
7896746 9.237066
7896748 7.955493
7896750 4.475332
7896752 9.805733
7896754 7.268561
7896756 5.409482
7896759 6.51506
7896761 7.942954
7896779 7.126118
7896817 6.704871
7896822 7.068824
7896859 6.305852
7896861 3.819883
7896863 5.417332
7896865 7.350156
7896878 7.112662

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.

Supplementary file Size Download File type/resource
GSM2340113_PNTAP-KONG-3_HuGene-1_0-st-v1_.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap