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Sample GSM2340112 Query DataSets for GSM2340112
Status Public on Oct 09, 2019
Title HEK293T transfected with pNTAP-2×flag empty vector, biological replicate 2
Sample type RNA
Source name Gene expression data from HEK293T transfected with pNTAP-2xflag vector
Organism Homo sapiens
Characteristics cell line: HEK293T
transfection: pNTAP-2×flag empty vector
Treatment protocol Total RNA was isolated from cells 24 hours after transfection using TRIzol reagent (Invitrogen).
Growth protocol The full-length coding sequence of pig VRTN was subcloned into pNTAP-2×flag expression vectors. HEK293T cells were transfected with pNTAP-2×flag-VRTN or empty vectors.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip2 Scanner 3000 7G.
Data processing Netaffx build 36 was used for the analysis. The data were analyzed with Affymetrix Expression Console Software (version 1.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Oct 08, 2016
Last update date Oct 09, 2019
Contact name Yanyu Dean
Phone 86-791-83813080
Organization name Jiangxi Agricultural University
Lab State Key Laboratory of Pig Genetic Improvement and Production Technology
Street address Lushanzhong888, Changbei district
City Nanchang
ZIP/Postal code 330044
Country China
Platform ID GPL6244
Series (2)
GSE87772 Expression data from HEK293T cells transfected with pNTAP-2×flag-VRTN or empty vectors
GSE87776 VRTN Regulates the Number of Thoracic Vertebrae During Vertebrate Development

Data table header descriptions
VALUE log2 GC-RMA signal

Data table
7896738 3.263564
7896740 3.217437
7896742 8.663717
7896744 4.641555
7896746 9.123989
7896748 8.59773
7896750 4.605779
7896752 9.798888
7896754 7.307176
7896756 5.163069
7896759 6.820661
7896761 8.009804
7896779 7.162155
7896817 6.755746
7896822 7.127343
7896859 6.286526
7896861 4.182372
7896863 5.761346
7896865 7.484946
7896878 7.218431

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.

Supplementary file Size Download File type/resource
GSM2340112_PNTAP-KONG-2_HuGene-1_0-st-v1_.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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