|
| Status |
Public on Oct 09, 2019 |
| Title |
HEK293T transfected with pNTAP-2×flag-VRTN, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Gene expression data from HEK293T transfected with pNTAP-2xflag-VRTN vector
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293T
transfection: pNTAP-2×flag-VRTN
|
| Treatment protocol |
Total RNA was isolated from cells 24 hours after transfection using TRIzol reagent (Invitrogen).
|
| Growth protocol |
The full-length coding sequence of pig VRTN was subcloned into pNTAP-2×flag expression vectors. HEK293T cells were transfected with pNTAP-2×flag-VRTN or empty vectors.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip2 Scanner 3000 7G.
|
| Data processing |
Netaffx build 36 was used for the analysis. The data were analyzed with Affymetrix Expression Console Software (version 1.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 08, 2016 |
| Last update date |
Oct 09, 2019 |
| Contact name |
Yanyu Dean |
| E-mail(s) |
yanyuduan@gmail.com
|
| Phone |
86-791-83813080
|
| Organization name |
Jiangxi Agricultural University
|
| Lab |
State Key Laboratory of Pig Genetic Improvement and Production Technology
|
| Street address |
Lushanzhong888, Changbei district
|
| City |
Nanchang |
| ZIP/Postal code |
330044 |
| Country |
China |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE87772 |
Expression data from HEK293T cells transfected with pNTAP-2×flag-VRTN or empty vectors |
| GSE87776 |
VRTN Regulates the Number of Thoracic Vertebrae During Vertebrate Development |
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