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Sample GSM2340109 Query DataSets for GSM2340109
Status Public on Oct 09, 2019
Title HEK293T transfected with pNTAP-2×flag-VRTN, biological replicate 2
Sample type RNA
Source name Gene expression data from HEK293T transfected with pNTAP-2xflag-VRTN vector
Organism Homo sapiens
Characteristics cell line: HEK293T
transfection: pNTAP-2×flag-VRTN
Treatment protocol Total RNA was isolated from cells 24 hours after transfection using TRIzol reagent (Invitrogen).
Growth protocol The full-length coding sequence of pig VRTN was subcloned into pNTAP-2×flag expression vectors. HEK293T cells were transfected with pNTAP-2×flag-VRTN or empty vectors.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip2 Scanner 3000 7G.
Data processing Netaffx build 36 was used for the analysis. The data were analyzed with Affymetrix Expression Console Software (version 1.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Oct 08, 2016
Last update date Oct 09, 2019
Contact name Yanyu Dean
Phone 86-791-83813080
Organization name Jiangxi Agricultural University
Lab State Key Laboratory of Pig Genetic Improvement and Production Technology
Street address Lushanzhong888, Changbei district
City Nanchang
ZIP/Postal code 330044
Country China
Platform ID GPL6244
Series (2)
GSE87772 Expression data from HEK293T cells transfected with pNTAP-2×flag-VRTN or empty vectors
GSE87776 VRTN Regulates the Number of Thoracic Vertebrae During Vertebrate Development

Data table header descriptions
VALUE log2 GC-RMA signal

Data table
7896738 2.954882
7896740 2.903116
7896742 8.446438
7896744 4.7482
7896746 9.22986
7896748 9.466896
7896750 4.416998
7896752 9.808182
7896754 7.357947
7896756 5.079651
7896759 6.283735
7896761 8.037586
7896779 7.039005
7896817 6.751734
7896822 7.239226
7896859 6.240099
7896861 3.794479
7896863 5.312267
7896865 7.359591
7896878 7.303959

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.

Supplementary file Size Download File type/resource
GSM2340109_VRTN-CDS-2_HuGene-1_0-st-v1_.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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