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Sample GSM2335350 Query DataSets for GSM2335350
Status Public on Apr 04, 2017
Title 19-UC-L3-2
Sample type RNA
 
Source name dorsolateral prefrontal cortex
Organism Homo sapiens
Characteristics tissue: dorsolateral prefrontal cortex
cell type: pyramidal neuron cell
shape: characteristic triangular shape and prominent apical dendrite
genotype: Unaffected Comparison subject
dsm-iv diagnoses: Unaffected Comparison
Treatment protocol An independent committee of experienced research clinicians made consensus DSM-IV diagnoses for each subject using information obtained from medical records and structured diagnostic interviews conducted with the decedent’s family members.
Growth protocol Brain specimens were obtained during autopsies conducted at the Allegheny County Office of the Medical Examiner (Pittsburgh, PA) after consent was obtained from the next-of-kin.
Extracted molecule total RNA
Extraction protocol For each sample, RNA was extracted using the QIAGEN® RNeasy Plus Micro kit (QIAGEN, Valencia).
Label Encore Biotin module (Nugen)
Label protocol Labeled using the Encore Biotin module (Nugen).
 
Hybridization protocol CDNA was synthesized and amplified using the Ovation Pico WTA System.
Scan protocol Loaded on an Affymetrix GeneChip® U219.
Description Gene expression data for subject 19, diagnosis UC, layer 3, replicate 2
Data processing Expression intensities were extracted from Affymetrix Expression Console and normalized using Robust Multi-array Average Express.
 
Submission date Oct 04, 2016
Last update date Apr 04, 2017
Contact name David Lewis
E-mail(s) lewisda@upmc.edu
Organization name University of Pittsburgh
Department Psychiatry and Neuroscience
Street address 3811 O'Hara St
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
 
Platform ID GPL13667
Series (1)
GSE87610 Gene expression of L3 and L5 pyramidal neurons in the DLPFC comparing schizophrenia from bipolar major depressive disorders and unaffected subjects.

Data table header descriptions
ID_REF
VALUE Background corrected, log2 transformed and then quantile normalized gene expression intensity.

Data table
ID_REF VALUE
AFFX-DapX-5_at 2.457257
AFFX-DapX-M_at 2.384909
AFFX-DapX-3_at 2.704998
AFFX-LysX-5_at 1.961286
AFFX-LysX-M_at 2.278694
AFFX-LysX-3_at 1.956054
AFFX-PheX-5_at 2.122544
AFFX-PheX-M_at 2.473207
AFFX-PheX-3_at 2.645741
AFFX-ThrX-5_at 2.345066
AFFX-ThrX-M_at 2.286506
AFFX-ThrX-3_at 2.904002
AFFX-TrpnX-5_at 1.942381
AFFX-TrpnX-M_at 2.311319
AFFX-TrpnX-3_at 2.116844
AFFX-r2-Ec-bioB-5_at 8.223227
AFFX-r2-Ec-bioB-M_at 8.362556
AFFX-r2-Ec-bioB-3_at 8.357052
AFFX-r2-Ec-bioC-5_at 9.420938
AFFX-r2-Ec-bioC-3_at 9.423576

Total number of rows: 49386

Table truncated, full table size 1081 Kbytes.




Supplementary file Size Download File type/resource
GSM2335350_5500994192207072414571_A04.cel.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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