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Sample GSM2335331 Query DataSets for GSM2335331
Status Public on Apr 04, 2017
Title 37-BP-L5-3
Sample type RNA
 
Source name dorsolateral prefrontal cortex
Organism Homo sapiens
Characteristics tissue: dorsolateral prefrontal cortex
cell type: pyramidal neuron cell
shape: characteristic triangular shape and prominent apical dendrite
genotype: Bipolar
dsm-iv diagnoses: Bipolar I, PF
Treatment protocol An independent committee of experienced research clinicians made consensus DSM-IV diagnoses for each subject using information obtained from medical records and structured diagnostic interviews conducted with the decedent’s family members.
Growth protocol Brain specimens were obtained during autopsies conducted at the Allegheny County Office of the Medical Examiner (Pittsburgh, PA) after consent was obtained from the next-of-kin.
Extracted molecule total RNA
Extraction protocol For each sample, RNA was extracted using the QIAGEN® RNeasy Plus Micro kit (QIAGEN, Valencia).
Label Encore Biotin module (Nugen)
Label protocol Labeled using the Encore Biotin module (Nugen).
 
Hybridization protocol CDNA was synthesized and amplified using the Ovation Pico WTA System.
Scan protocol Loaded on an Affymetrix GeneChip® U219.
Description Gene expression data for subject 37, diagnosis BP, layer 5, replicate 1
Data processing Expression intensities were extracted from Affymetrix Expression Console and normalized using Robust Multi-array Average Express.
 
Submission date Oct 04, 2016
Last update date Apr 04, 2017
Contact name David Lewis
E-mail(s) lewisda@upmc.edu
Organization name University of Pittsburgh
Department Psychiatry and Neuroscience
Street address 3811 O'Hara St
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
 
Platform ID GPL13667
Series (1)
GSE87610 Gene expression of L3 and L5 pyramidal neurons in the DLPFC comparing schizophrenia from bipolar major depressive disorders and unaffected subjects.

Data table header descriptions
ID_REF
VALUE Background corrected, log2 transformed and then quantile normalized gene expression intensity.

Data table
ID_REF VALUE
AFFX-DapX-5_at 3.752084
AFFX-DapX-M_at 4.034998
AFFX-DapX-3_at 5.196601
AFFX-LysX-5_at 2.271024
AFFX-LysX-M_at 2.526229
AFFX-LysX-3_at 2.96431
AFFX-PheX-5_at 2.514842
AFFX-PheX-M_at 2.793586
AFFX-PheX-3_at 3.469547
AFFX-ThrX-5_at 2.76889
AFFX-ThrX-M_at 3.179011
AFFX-ThrX-3_at 4.563633
AFFX-TrpnX-5_at 2.112351
AFFX-TrpnX-M_at 2.256905
AFFX-TrpnX-3_at 2.159557
AFFX-r2-Ec-bioB-5_at 8.759331
AFFX-r2-Ec-bioB-M_at 8.895617
AFFX-r2-Ec-bioB-3_at 8.662165
AFFX-r2-Ec-bioC-5_at 9.777649
AFFX-r2-Ec-bioC-3_at 9.716311

Total number of rows: 49386

Table truncated, full table size 1081 Kbytes.




Supplementary file Size Download File type/resource
GSM2335331_5500994192207072414571_H04.cel.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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